Arylamines Suppress Their Own Activation and That of Nitroarenes in V79 Chinese Hamster Cells by Competing for Acetyltransferases
| Autor: | Rashmeet K. Reen, Olga Cumpelik, Friedrich J. Wiebel, Franz Kiefer, Johannes Doehmer |
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| Rok vydání: | 1994 |
| Předmět: |
Health
Toxicology and Mutagenesis CHO Cells Binding Competitive Chinese hamster In vivo Acetyltransferases Cricetinae Cytotoxic T cell Animals Cytotoxicity Carcinogen Anthracenes Fluorenes Pyrenes biology Chemistry Chinese hamster ovary cell Public Health Environmental and Occupational Health respiratory system biology.organism_classification Molecular biology Biochemistry Acetyltransferase Micronucleus test Carcinogens Drug Antagonism Research Article Mutagens |
| Zdroj: | Environmental Health Perspectives |
| ISSN: | 0091-6765 |
| DOI: | 10.2307/3432159 |
| Popis: | The effect of 2-aminofluorene (2-AF) on the toxicity of 2-aminoanthracene (2-AA) and 1,6-dinitropyrene (1,6-DNP) was studied in N-acetyltransferase-proficient V79-NHr1A2 cells genetically engineered for the expression of cytochrome P4501A2, and in wild-type V79-NH cells. 2-AA inhibited the growth of V79-NHr1A2 cells and induced the formation of micronuclei at concentrations of 0.1 to 1.0 microM, but was virtually without toxic effects at a concentration of 10 microM. Addition of 2-AF protected against the cytotoxic and genotoxic effects elicited by low concentrations of 2-AA. Half-maximum protection was observed at 0.2 to 0.5 microM 2-AF. The arylamine also prevented the cytotoxicity caused by 1,6-DNP in V79-NH cells and completely suppressed the formation of 1-acetylamino-6-nitropyrene from 1,6-DNP in these cells. The results indicate that arylamines and related N-hydroxyarylamines are substrates for the same acetyltransferase in V79-NH cells. In consequence, arylamines are capable of suppressing the activation of their proximate cytotoxic and genotoxic products in these cells and, presumably, in vivo. |
| Databáze: | OpenAIRE |
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