Early Colonization of Lactobacillus reuteri after Exposure to Probiotics
Autor: | Nursen Topcuoglu, Nuket Sandalli, Güven Külekçi, Esber Caglar, H Ozbey |
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Přispěvatelé: | Çaglar, E., Topcuoglu, N., Ozbey, H., Sandalli, N., Kulekci, G., Yeditepe Üniversitesi |
Rok vydání: | 2015 |
Předmět: |
Limosilactobacillus reuteri
Saliva Probiotic bacteria Lactobacillus reuteri Biology law.invention Microbiology Rats Sprague-Dawley Streptococcus mutans Random Allocation Probiotic law Animals Animal study Colonization Anaerobiosis Bacteriological Techniques Mouth Probiotics Age Factors General Medicine Standard methods biology.organism_classification Bacterial Load Rats Oral microbiology Rat Female |
Zdroj: | Journal of Clinical Pediatric Dentistry. 39:326-330 |
ISSN: | 1557-5268 1053-4628 |
DOI: | 10.17796/1053-4628-39.4.326 |
Popis: | The aim of the present in vivo animal study is to investigate the ability of L. reuteri to colonize the oral flora during infancy. Study design: Twenty four rats, aged 1 month, which were pre-analyzed for mutans streptococci and L. reuteri absence in their saliva, were randomly divided into 3 groups. The control group was infected with S. mutans ATCC 25175 at the 2nd month, three times a day for 14 days. S. mutans counts were determined with microbiological saliva analyzes obtained by standard methods of oral swabbing at 3rd, 4th and 5th months. The second group, Probiotic I group, was also infected with S. mutans at the 2nd month, and further infected with L. reuteri ATCC 55730 (1×108),5 drops per day for 25 days, at the 3rd month. S. mutans and L. reuteri counts were determined at the 3rd, 4th and 5th months. Plates were incubated anaerobically at 37°C for 48 h, after which colonies were confirmed as L. Reuteri. Results: Regarding intragroup analysis, S. mutans counts of the Control group increased steadily during the 3rd. and 4th. months, and a statistically significant (p |
Databáze: | OpenAIRE |
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