Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
| Autor: | Ignacia Braga Tanaka, Chihiro Harada, Yoshiko Ayabe, Jun-ichiro Komura, Keiji Ogura, Satoshi Tanaka |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
DNA Copy Number Variations Offspring QH301-705.5 Longevity low dose-rate Male mice Biology Radiation Dosage Genome Article Catalysis Ionizing radiation Inorganic Chemistry Andrology Mice Pregnancy Animals Low dose rate Copy-number variation Irradiation Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy Comparative Genomic Hybridization transgenerational effects Organic Chemistry array comprehensive genomic hybridization (array CGH) General Medicine Computer Science Applications Mice Inbred C57BL Chemistry copy number variation (CNV) Gamma Rays Prenatal Exposure Delayed Effects Paternal Exposure Female genetic effects ionizing radiation Comparative genomic hybridization |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 12437, p 12437 (2021) International Journal of Molecular Sciences Volume 22 Issue 22 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridization technology. We observed that the number of the mice with de novo copy number variations (specifically, deletions) was significantly increased in the offspring of C57BL/6J male mice exposed to 20 mGy/day gamma-rays for 400 days (total dose: 8000 mGy), as compared to non-irradiated controls. We did not detect any difference in the size of the de novo deletions between the irradiated and the non-irradiated groups. An analysis of the life span of the offspring suggested a possibility that de novo copy-number variations may be associated with shorter life spans. |
| Databáze: | OpenAIRE |
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