A longitudinal study of the infant nasopharyngeal microbiota: The effects of age, illness and antibiotic use in a cohort of South East Asian children
| Autor: | Claudia Turner, Julian Parkhill, Susannah J. Salter, Paul Turner, Marcus C. de Goffau, Stephen D. Bentley, Josef Wagner, François Nosten, David Goldblatt, Wanitda Watthanaworawit |
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| Přispěvatelé: | Salter, Susannah J [0000-0003-3898-8504], Watthanaworawit, Wanitda [0000-0001-5313-8319], Parkhill, Julian [0000-0002-7069-5958], Turner, Paul [0000-0002-1013-7815], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Longitudinal study Pulmonology Staphylococcus medicine.disease_cause Pathology and Laboratory Medicine Cohort Studies Families Antibiotics Nasopharynx RNA Ribosomal 16S Moraxellaceae Medicine and Health Sciences Longitudinal Studies Children Respiratory Tract Infections education.field_of_study Refugees biology Antimicrobials lcsh:Public aspects of medicine Microbiota Age Factors Respiratory infection Drugs Pneumococcus Genomics 3. Good health Bacterial Pathogens Anti-Bacterial Agents Infectious Diseases Streptococcus pneumoniae Medical Microbiology Cohort Carrier State Ornithobacterium Female Pathogens Infants Research Article medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 030106 microbiology Population Microbial Genomics Microbiology 03 medical and health sciences Internal medicine Microbial Control medicine Genetics Humans education Moraxella Microbial Pathogens Pharmacology Bacteria Public Health Environmental and Occupational Health Organisms Infant Newborn Biology and Life Sciences Streptococcus Infant Corynebacteria lcsh:RA1-1270 Pneumonia biology.organism_classification Flavobacteriaceae 030104 developmental biology Age Groups Immunology People and Places Population Groupings Microbiome |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0005975 (2017) PLoS Neglected Tropical Diseases |
| Popis: | A longitudinal study was undertaken in infants living in the Maela refugee camp on the Thailand-Myanmar border between 2007 and 2010. Nasopharyngeal swabs were collected monthly, from birth to 24 months of age, with additional swabs taken if the infant was diagnosed with pneumonia according to WHO clinical criteria. At the time of collection, swabs were cultured for Streptococcus pneumoniae and multiple serotype carriage was assessed. The bacterial 16S rRNA gene profiles of 544 swabs from 21 infants were analysed to see how the microbiota changes with age, respiratory infection, antibiotic consumption and pneumococcal acquisition. The nasopharyngeal microbiota is a somewhat homogenous community compared to that of other body sites. In this cohort it is dominated by five taxa: Moraxella, Streptococcus, Haemophilus, Corynebacterium and an uncharacterized Flavobacteriaceae taxon of 93% nucleotide similarity to Ornithobacterium. Infant age correlates with certain changes in the microbiota across the cohort: Staphylococcus and Corynebacterium are associated with the first few months of life while Moraxella and the uncharacterised Flavobacteriaceae increase in proportional abundance with age. Respiratory illness and antibiotic use often coincide with an unpredictable perturbation of the microbiota that differs from infant to infant and in different illness episodes. The previously described interaction between Dolosigranulum and Streptococcus was observed in these data. Monthly sampling demonstrates that the nasopharyngeal microbiota is in flux throughout the first two years of life, and that in this refugee camp population the pool of potential bacterial colonisers may be limited. Author summary The nasopharynx hosts a community of microbes that first colonise us during infancy and that changes as we grow. Colonisation with certain species is a risk factor for developing respiratory infections such as pneumonia, while other species can have a protective influence. In this study we use molecular methods to identify the bacteria present in nasopharyngeal swabs taken regularly from children in a refugee camp in Thailand. The microbiota develops with age, with early colonisers such as Corynebacterium or Staphylococcus being eventually outgrown by Moraxella and an uncultured taxon described here as unclassified Flavobacteriaceae I. There is evidence in the cohort of Streptococcus pneumoniae being frequently carried and transmitted throughout the first two years of life. We found that the microbiota profiles were not unique or distinguishable between individuals in this study, which is unlike studies in high income, low density populations. |
| Databáze: | OpenAIRE |
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