Isolation, identification and selection of bacteria with the proof-of-concept for bioaugmentation of whitewater from wood-free paper mills
| Autor: | Ziga Veliscek, Anja Erzin, Aleš Lapanje, Iaroslav Rybkin, Tomaz Rijavec, Nada Verdel, Albin Pintar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Bioaugmentation
PCA Aeromonas sp biology Papermaking Pilot scale Cellulosimicrobium sp Cellulosimicrobium water treatment biology.organism_classification Isolation (microbiology) Pulp and paper industry Polyvinyl alcohol chemistry.chemical_compound chemistry azo dye Aeromonas bioaugmentation Bacteria |
| Zdroj: | Frontiers in Microbiology 12(2021), 758702 |
| Popis: | When whitewater circuits in the paper industry are closed, organic compounds accumulate and cause adverse production problems, such as the formation of slime and pitch. Since wood-free whitewater is usually a mixture of additives for paper production and an efficient cost-effective purification technology for their removal is lacking, the aim of our study was to find an effective bio-based strategy for whitewater treatment using a selection of indigenous bacterial isolates. We first obtained a large collection of bacterial isolates and then tested them individually for their ability to degrade the organic additives used in papermaking, i.e. carbohydrates, resin acids, alkyl ketene dimers, polyvinyl alcohol, latex, and azo and fluorescent dyes. Out of the 355 bacterial isolates, we selected a combination of four strains (Xanthomonadales bacterium sp. CST37-CF, Sphingomonas sp. BLA14-CF, Cellulosimicrobium sp. AKD4-BF and Aeromonas sp. RES19-BTP), which cover the entire spectrum of the tested organic additives. A proof-of-concept study in pilot scale was then performed by immobilizing the cells of our artificial bacterial consortium in a 33-liter tubular flow-through reactor with a retention time of Graphical abstractHighlightsA strategy for selecting a consortium of indigenous bacteria with a high potential for bioaugmentation of wood-free whitewater is presented.Study of bacterial degradation of eight chemically diverse substances used in paper production is presented. Methods for the selection of bacteria suitable for industrial application were developed.The constructed artificial bacterial consortium consisted of strains belonging to genera Xanthomonadales bacterium, Sphingomonas, Aeromonas and Cellulosimicrobium.The proof of concept of the industrial application, consisting of a 33-L-column filled with the immobilized artificial consortium, was an 88% decrease in COD of the whitewater effluent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|