Two types of interneurons in the mouse lateral geniculate nucleus are characterized by different h-current density
| Autor: | Maia Datunashvilli, Michael Leist, Mehrnoush Zobeiri, Ania Aissaoui, Thomas Budde, Hans-Christian Pape, Tatyana Kanyshkova, Maria Novella Romanelli, Manuela Cerina |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Gene isoform Multidisciplinary h-current drug design Thalamus Action Potentials Biology Lateral geniculate nucleus Article 03 medical and health sciences Bursting Mice 0302 clinical medicine Interneurons Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Animals GABAergic Neurons Membrane potential Multidisciplinary Geniculate Bodies Depolarization Anatomy Electrophysiology 030104 developmental biology Biophysics GABAergic 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Although hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channels and the corresponding h-current (Ih) have been shown to fundamentally shape the activity pattern in the thalamocortical network, little is known about their function in local circuit GABAergic interneurons (IN) of the dorsal part of the lateral geniculate nucleus (dLGN). By combining electrophysiological, molecular biological, immunohistochemical and cluster analysis, we characterized the properties of Ih and the expression profile of HCN channels in IN. Passive and active electrophysiological properties of IN differed. Two subclasses of IN were resolved by unsupervised cluster analysis. Small cells were characterized by depolarized resting membrane potentials (RMP), stronger anomalous rectification, higher firing frequency of faster action potentials (APs), appearance of rebound bursting, and higher Ih current density compared to the large IN. The depolarization exerted by sustained HCN channel activity facilitated neuronal firing. In addition to cyclic nucleotides, Ih in IN was modulated by PIP2 probably based on the abundant expression of the HCN3 isoform. Furthermore, only IN with larger cell diameters expressed neuronal nitric oxide synthase (nNOS). It is discussed that Ih in IN is modulated by neurotransmitters present in the thalamus and that the specific properties of Ih in these cells closely reflect their modulatory options. |
| Databáze: | OpenAIRE |
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