Generation and Application of Monoclonal Antibody Against Lycopene
| Autor: | Elena Y. Morgunova, Nigel H. Kyle, Ivan M. Petyaev, Natalya E. Chalyk, Yuriy K. Bashmakov, Marina V. Lozbiakova, Valeriy V. Tsibezov, Alexey Y. Kozlov, Dmitry V. Pristenskiy, Marina P. Chernyshova, Naylia A. Zigangirova, L. V. Kostina |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Immunogen Immunoconjugates medicine.drug_class medicine.medical_treatment Immunology Blotting Western Freund's Adjuvant Immunization Secondary Enzyme-Linked Immunosorbent Assay Gold Colloid Monoclonal antibody 03 medical and health sciences chemistry.chemical_compound Mice Lycopene Antigen Antibody Specificity Macrophages Alveolar medicine Immunology and Allergy Animals Humans Antigens Fluorescent Antibody Technique Indirect Carotenoid Cells Cultured chemistry.chemical_classification Mice Inbred BALB C Hybridomas biology Chemistry Carotene Lutein Antibodies Monoclonal Carotenoids 030104 developmental biology Biochemistry Freund's adjuvant biology.protein Hepatocytes Antibody Spleen |
| Zdroj: | Monoclonal antibodies in immunodiagnosis and immunotherapy. 36(2) |
| ISSN: | 2167-9436 |
| Popis: | A monoclonal antibody (Mab) against lycopene was developed from hybridoma clones obtained from BALB/c mice immunized with trans-isomer of lycopene (t-lycopene, t-LC) conjugated with colloidal gold particles. An alternating immunization schedule which included injection of both formulations of immunogen (without and with Freund's adjuvant) was most effective in the elucidation of a measurable immune response to the t-Lycopene conjugate. Selected hybridoma clones were able to produce an Mab positive in competition assay. In particular, preincubation of 6B9 Mabs with t-LC abolished the ability of 6B9 Mabs to bind LC in the competition assay. Mabs produced by other clones (4F10, 4A3, and 3B12) worked similarly. Analysis of antigen specificity showed that 6B9 Mab raised against t-LC did not recognize other carotenoids such as lutein and carotene. Mab 6B9 was shown to recognize lycopene on a glass surface and in the settings of indirect immunofluorescence experiments performed in cultured hepatocytes and alveolar macrophages incubated with and without lycopene, as well as in sebum and corneocyte specimens from the skin of volunteers supplemented with nutraceutical formulation of lycopene. Newly generated Mabs against lycopene may provide a valuable tool for different analytical assays of lycopene content in various biological, agricultural, and food products. |
| Databáze: | OpenAIRE |
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