PARK7/DJ-1 inhibition decreases invasion and proliferation of uveal melanoma cells
| Autor: | Nerea Lago-Baameiro, Maria Santiago-Varela, Tamara Camino, Paula Silva-Rodríguez, Manuel Bande, Maria Jose Blanco-Teijeiro, Maria Pardo, Antonio Piñeiro |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Tumori Journal. 109:47-53 |
| ISSN: | 2038-2529 0300-8916 |
| Popis: | Introduction: PARK7/DJ-1 is an oncogene that is associated with tumorigenesis in many cancers. Recent studies have demonstrated the importance of DJ-1 in the origin and development of uveal melanoma (UM). We present an analysis of the role of the DJ-1 protein in UM cells, especially in its effect on proliferation and migration. Methods: UM cells from a primary tumor, Mel 270, and its liver metastasis, OMM2.5, were transfected with lentiviral-delivered shRNA against PARK7/DJ-1. Evaluation of cell migration and proliferation was performed using the xCELLigence real-time cell analyzer (RTCA). The effect of DJ-1 inhibition on the PTEN-Akt signaling pathway was also studied by immunoblotting. Results: The silencing of PARK7/DJ-1 oncoprotein expression produced a significant decrease of phosphorylated Akt (S473) in Mel270 and in metastatic OMM2.5 UM cells with no alteration on tumor suppressor PTEN expression. The diminution of PARK7/DJ-1 expression significantly inhibited real-time proliferation and invasion of Mel270 and OMM2.5 and the invasion potential of the metastatic cells. Conclusion: DJ-1 appears to play a key role on the PTEN/Akt pathway in UM. DJ-1 inhibition appears to have a negative effect on proliferation and invasion of UM cells. This suggests DJ-1 as a potential therapeutic target in UM. |
| Databáze: | OpenAIRE |
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