Green tea compound epigallo-catechin-3-gallate (EGCG) increases neuronal survival in adult hippocampal neurogenesis in vivo and in vitro
| Autor: | R.I. Téllez-Ballesteros, Mario O. Torres-Pérez, Gerardo Ramírez-Rodríguez, Leonardo Ortiz-López, Gerd Kempermann, Muhammad Ichwan, Ariadna Gómez-Sánchez, M.D.C. Silva-Lucero, Berenice Márquez-Valadez, Marco Antonio Meraz-Ríos |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry [Tea]
0301 basic medicine Doublecortin Domain Proteins Male Neural precursor cell proliferation drug effects [Hippocampus] drug effects [Neural Stem Cells] metabolism [Neuropeptides] physiology [Hippocampus] drug effects [Neurogenesis] Hippocampal formation Hippocampus Catechin Phosphatidylinositol 3-Kinases 0302 clinical medicine Neural Stem Cells physiology [Neural Stem Cells] heterocyclic compounds Cells Cultured Neurons education.field_of_study Mice Inbred BALB C General Neuroscience Neurogenesis analogs & derivatives [Catechin] food and beverages physiology [Neurogenesis] physiology [Neurons] Neural stem cell Cell biology chemistry [Catechin] Neuroprotective Agents chemistry [Neuroprotective Agents] metabolism [Proto-Oncogene Proteins c-akt] pharmacology [Catechin] Microtubule-Associated Proteins epigallocatechin gallate Doublecortin Protein Cell Survival drug effects [Cell Survival] physiology [Cell Survival] Population Biology complex mixtures 03 medical and health sciences Precursor cell drug effects [Neurons] Animals ddc:610 education metabolism [Phosphatidylinositol 3-Kinases] PI3K/AKT/mTOR pathway Dose-Response Relationship Drug Tea pharmacology [Neuroprotective Agents] Dentate gyrus Neuropeptides metabolism [Microtubule-Associated Proteins] 030104 developmental biology sense organs Neuroscience Proto-Oncogene Proteins c-akt 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience 322, 208-220 (2016). doi:10.1016/j.neuroscience.2016.02.040 |
| ISSN: | 1873-7544 |
| DOI: | 10.1016/j.neuroscience.2016.02.040 |
| Popis: | Epigallo-catechin-3-gallate (EGCG), found in the leaves of Camellia sinensis (green tea), has antioxidant- and scavenger-functions and acts neuroprotectively. It has been publicized as anti-aging remedy but data on potential cellular mechanisms are scarce. Recent studies claimed that EGCG specifically promotes neural precursor cell proliferation in the dentate gyrus of C57Bl/6 mice, without changes at the level of immature and mature new neurons. We here analyzed the effects of EGCG on adult hippocampal neurogenesis in male Balb/C mice and saw a different pattern. Two weeks of treatment with EGCG (0, 0.625, 1.25, 2.5, 5 and 10mg/kg) showed a dose-response curve that peaked at 2.5mg/kg of EGCG with significantly increased cell survival without affecting cell proliferation but decreasing apoptotic cells. Also, EGCG increased the population of doublecortin-(DCX)-expressing cells that comprises the late intermediate progenitor cells (type-2b and -3) as well as immature neurons. After EGCG treatment, the young DCX-positive neurons showed more elaborated dendritic trees. EGCG also significantly increased net neurogenesis in the adult hippocampus and increased the hippocampal levels of phospho-Akt. Ex vivo, EGCG exerted a direct effect on survival and neuronal differentiation of adult hippocampal precursor cells, which was absent, when PI3K, a protein upstream of Akt, was blocked. Our results thus support a pro-survival and a pro-neurogenic role of EGCG. In the context of the conflicting published results, however, potential genetic modifiers must be assumed. These might help to explain the overall variability of study results with EGCG. Our data do indicate, however, that natural compounds such as EGCG can in principle modulate brain plasticity. |
| Databáze: | OpenAIRE |
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