High abundance of sugar metabolisers in saliva of children with caries
| Autor: | Sajan C. Raju, Catharina Sarkkola, Heli Viljakainen, Muhammed A.P. Manzoor, Sohvi Lommi, Elina Engberg, Jussi Furuholm |
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| Přispěvatelé: | Department of Public Health, University of Helsinki, HUS Head and Neck Center, Department of Oral and Maxillofacial Diseases, Faculty of Medicine, Medicum, Helsinki Inequality Initiative (INEQ), Department of Psychology and Logopedics, HUS Children and Adolescents, Department of Food and Nutrition |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Saliva Dental Caries Susceptibility Science Dentistry Microbial communities Diseases Oral health Dental Caries Article 03 medical and health sciences 0302 clinical medicine Medical research 3123 Gynaecology and paediatrics RNA Ribosomal 16S medicine Cavitated caries Sequencing Humans Clinical significance Child 2. Zero hunger Multidisciplinary business.industry Dental health Microbiota 030206 dentistry medicine.disease 313 Dentistry Dentition Permanent stomatognathic diseases 030104 developmental biology Risk factors Bacterial 16S rRNA Potential biomarkers Biofilms Medicine Dysbiosis Female Pathogens business Sugars |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Dental caries is a biofilm-mediated, dynamic disease with early onset. A balanced salivary microbiota is a foundation of oral health, while dysbiosis causes tooth decay. We compared the saliva microbiota profiles in children with and without caries. The study consisted of 617 children aged 9–12 years from the Finnish Health in Teens (Fin-HIT) study with available register data on oral health. Caries status was summarised based on Decayed, Missing, and Filled Teeth (DMFT) index in permanent dentition. The children were then classified into the following two groups: DMFT value ≥ 1 was considered as cavitated caries lesions (hereafter called ‘caries’) (n = 208) and DMFT = 0 as ‘cavity free’ (n = 409). Bacterial 16S rRNA gene (V3–V4 regions) was amplified using PCR and sequenced by Illumina HiSeq. The mean age (SD) of the children was 11.7 (0.4) years and 56% were girls. The children had relatively good dental health with mean DMFT of 0.86 (1.97). Since sex was the key determinant of microbiota composition (p = 0.014), we focused on sex-stratified analysis. Alpha diversity indexes did not differ between caries and cavity free groups in either sexes (Shannon: p = 0.40 and 0.58; Inverse Simpson: p = 0.51 and 0.60, in boys and girls, respectively); neither did the composition differ between the groups (p = 0.070 for boys and p = 0.230 for girls). At the genus level, Paludibacter and Labrenzia had higher abundances in the caries group compared to cavity free group in both sexes (p Paludibacter and Labrenzia. These bacteria presumably enhance salivary acidification, which contributes to progression of dental caries. The clinical relevance of our findings warrants further studies. |
| Databáze: | OpenAIRE |
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