TLR2 may influence the behavior of the malignant clone in B-CLL
| Autor: | Halina Antosz, Joanna Sajewicz, Barbara Marzec-Kotarska, Małgorzata Jargiełło-Baszak, Anna Dmoszynska, Jacek Baszak |
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| Rok vydání: | 2012 |
| Předmět: |
Lipopolysaccharides
Male Staphylococcus aureus Receptor expression Clone (cell biology) Receptors Antigen B-Cell Stimulation CD19 immune system diseases hemic and lymphatic diseases Humans Molecular Biology Aged Aged 80 and over biology Gene Expression Regulation Leukemic Interleukin-6 breakpoint cluster region Cell Biology Hematology Middle Aged Leukemia Lymphocytic Chronic B-Cell Toll-Like Receptor 2 Toll-Like Receptor 4 TLR2 TLR4 Cancer research biology.protein Molecular Medicine Female CD5 |
| Zdroj: | Blood Cells, Molecules, and Diseases. 49:32-40 |
| ISSN: | 1079-9796 |
| Popis: | B-cell receptor (BCR) and Toll-like receptor (TLR) stimulation and integration with signals from the pathogen or immune cells and their products determine the B-cell antibody response. Low expression of BCR is the hallmark of B lymphocytes in CLL, however little is known about the expression and function of TLR in B-CLL. We studied TLR2, TLR4, IL-6 and mIL-6Rα expression on mRNA and protein level in CD19+ subpopulation of normal lymphocytes and the CD19+CD5+ subpopulation from B-CLL. Experiments were performed on unstimulated and stimulated lymphocytes [Staphylococcus aureus Cowan I (SAC) and lipopolysaccharide (LPS) from Escherichia coli — TLR2- and TLR4-specific agonists, respectively]. We showed undetectable or low IL-6 expression, which seems to be specific for B-CLL lymphocytes. Induction of TLR4 mRNA upon LPS stimulation affected the expression of IL-6, but not of mIL-6Rα. Increased expression of TLR2 (MFI) after LPS and SAC stimulation did not correlate with mIL-6Rα receptor expression. B-CLL CD19+CD5+ lymphocytes showed a significant increase in TLR2 expression at the protein level after stimulation with SAC and LPS compared to normal CD19+ lymphocytes. TLR2 may influence the behaviour of the malignant clone in B-CLL. |
| Databáze: | OpenAIRE |
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