Neuropeptide Substance P induces mRNA expression and secretion of CXCL8 chemokine, and HDC in human umbilical cord blood mast cells

Autor: M.L. Castellani, Kepuraj D, Pio Conti, Giuliano Giorgio Cerulli, Stefano Tetè, De Amicis D, Pierluigi Antinolfi, Theoharis C. Theoharides, Mario Felaco, Salini, Orso C, Paolo Boscolo, Alessandro Caraffa, Chiara Conti, C. Ciampoli
Přispěvatelé: Castellani, Ml, Ciampoli, C, M., Felaco, Tetè, S, Conti, Cm, Salini, V, DE AMICIS, D, Orso, C, Antinolfi, Pl, Caraffa, A, Cerulli, G, Boscolo, P, Theoharides, Tc, Conti, P, Kepuraj, D
Rok vydání: 2008
Předmět:
medicine.medical_specialty
Chemokine
RT-PCR
Gene Expression
Inflammation
Substance P
Histidine Decarboxylase
Biology
DISEASE
Dexamethasone
Proinflammatory cytokine
chemistry.chemical_compound
Microscopy
Electron
Transmission
Internal medicine
medicine
Humans
Mast Cells
RNA
Messenger
Interleukin 8
OXIDATIVE STRESS
CXCL14
Calcimycin
Cells
Cultured
NEUROGENIC INFLAMMATION
MONONUCLEAR-CELLS
Dose-Response Relationship
Drug
Reverse Transcriptase Polymerase Chain Reaction
INFLAMMATORY RESPONSE
Interleukin-8
LUNG INFLAMMATION
IN-VITRO
General Medicine
Fetal Blood
ENDOTHELIAL-CELLS
Histidine decarboxylase
Cell biology
DIFFERENTIATION
Endocrinology
chemistry
INFLAMMATORY RESPONSE
ENDOTHELIAL-CELLS
IN-VITRO
NEUROGENIC INFLAMMATION
LUNG INFLAMMATION
MONONUCLEAR-CELLS
OXIDATIVE STRESS
RT-PCR
DISEASE
DIFFERENTIATION
biology.protein
medicine.symptom
Histamine
Zdroj: Scopus-Elsevier
ISSN: 1488-2353
DOI: 10.25011/cim.v31i6.4923
Popis: Purpose: Mast cells play an important role in innate and acquired immunity and are thought to be the cellular origin of most proteases and cytokines. Substance P (SP) and its receptor, NK-1R, play critical roles in immune regulation in human and animal models of inflammation. Methods: We used mature human cord blood mast cells (HCBMC) differentiated from cord blood CD34+ precursor activated with SP in culture. Results: Our data indicate that Substance P strongly activates mature HCBMC in releasing CXCL8 expression and secretion (Control: 1.200 ± 1.0; SP: 4.10 ± 0.90; P < 0.01). Moreover, in a RT-PCR, HCBMC expressed CXCL8 mRNA after Substance P activation. Since calcium ionophore A23187 is a pharmacological activator that raises cytosolic free calcium ion concentraion and stimulates mast cells in the production and secretion of proinflammatory compounds, it was used as positive control. In addition, we found that HCBMCs generate the transcription of histidine decarboxylase (HDC), the enzyme responsible for the generation of histamine from histidine, after SP treatment. Since CXCL8 is a member of the CXC chemokine subfamily with potent chemotactic activity and is a primary inflammatory cytokine we conclude that our results, obtained from HCBMC cultures, a good and valid model in vitro, support the concept that the neurogenic system modulates inflammatory events by Substance P-mediated HCBMC chemokine CXCL8 release. Conclusion: The expression, synthesis and release of CXCL8 suggest an increase of inflammatory process in vivo mediated by the recruitment and infiltration of inflammatory cells in inflamed tissues.
Databáze: OpenAIRE
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