Use of Nile Red as a long-wavelength fluorophore in dual-probe studies of ligand-protein interactions
| Autor: | Marc B. Brown, Tony E. Edmonds, Nichola J. Seare, James N. Miller |
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| Rok vydání: | 1993 |
| Předmět: |
Fluorophore
Sociology and Political Science Ligand binding assay Clinical Biochemistry Analytical chemistry Nile red Ligand (biochemistry) Biochemistry Fluorescence Protein–protein interaction Clinical Psychology Bimolecular fluorescence complementation chemistry.chemical_compound chemistry Biophysics Binding site Law Spectroscopy Social Sciences (miscellaneous) |
| Zdroj: | Journal of Fluorescence. 3:129-130 |
| ISSN: | 1573-4994 1053-0509 |
| DOI: | 10.1007/bf00862729 |
| Popis: | The long-wavelength fluorescence probe, Nile Red, has polarity-dependent fluorescence intensity and wavelength properties that can be used to monitor the binding of drugs and other ligands to plasma proteins such as albumin and α-1 acid glycoprotein. This paper shows that it can be used in tandem with another fluorescence probe, 1-anilinonaphthalene-8-sulfonic acid, to study two or more types of ligand binding sites simultaneously. Some ligands displace one or the other probe from the protein/dual-probe complex, other ligands displace both probes. In each case the resulting decrease in fluorescence can be used to estimate the numbers of binding sites and their association constants. |
| Databáze: | OpenAIRE |
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