Effect of metformin on the vascular and glucose metabolic actions of insulin in hypertensive rats
| Autor: | Marta Santuré, Maryse Pitre, André Marette, André Nadeau, Nathalie Gaudreault, Hélène Bachelard |
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| Rok vydání: | 2000 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Physiology medicine.medical_treatment Hemodynamics Blood Pressure Rats Inbred WKY Heart Rate Rats Inbred SHR Physiology (medical) Internal medicine Animals Insulin Medicine Infusions Intravenous Muscle Skeletal Pancreatic hormone Hepatology business.industry Gastroenterology Metabolism Blood flow Glucose clamp technique Metformin Rats Kinetics Glucose Blood pressure Endocrinology Regional Blood Flow Glucose Clamp Technique business medicine.drug |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 278:G682-G692 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.2000.278.5.g682 |
| Popis: | We investigated the long-term effect of metformin treatment on blood pressure, insulin sensitivity, and vascular responses to insulin in conscious spontaneously hypertensive rats (SHR). The rats were instrumented with intravascular catheters and pulsed Doppler flow probes to measure blood pressure, heart rate, and blood flow. Insulin sensitivity was assessed by the euglycemic hyperinsulinemic clamp technique. Two groups of SHR received metformin (100 or 300 mg ⋅ kg− 1 ⋅ day− 1) for 3 wk while another group of SHR and a group of Wistar Kyoto (WKY) rats were left untreated. We found that vasodilation of skeletal muscle and renal vasculatures by insulin is impaired in SHR. Moreover, a reduced insulin sensitivity was detected in vivo and in vitro in isolated soleus and extensor digitorum longus muscles from SHR compared with WKY rats. Three weeks of treatment with metformin improves the whole-body insulin-mediated glucose disposal in SHR but has no blood pressure-lowering effect and no influence on vascular responses to insulin (4 mU ⋅ kg− 1 ⋅ min− 1). An improvement in insulin-mediated glucose transport activity was detected in isolated muscles from metformin-treated SHR, but in the absence of insulin no changes in basal glucose transport activity were observed. It is suggested that part of the beneficial effect of metformin on insulin resistance results from a potentiation of the hormone-stimulating effect on glucose transport in peripheral tissues (mainly skeletal muscle). The results argue against a significant antihypertensive or vascular effect of metformin in SHR. |
| Databáze: | OpenAIRE |
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