Glucocorticoid receptor localization in human epidermal cells
| Autor: | D. Schmitt, Jacqueline Viac, Mireille Serres |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Langerhans cell Cellular differentiation Blotting Western Stratum granulosum Human skin Cell Separation Dermatology Biology Skin Diseases Receptors Glucocorticoid Glucocorticoid receptor Reference Values Internal medicine medicine Animals Humans Tissue Distribution Receptor Cells Cultured Corneocyte integumentary system General Medicine Flow Cytometry Immunohistochemistry Molecular biology Endocrinology medicine.anatomical_structure Epidermal Cells Rabbits Epidermis Keratinocyte |
| Zdroj: | Archives of Dermatological Research. 288:140-146 |
| ISSN: | 1432-069X 0340-3696 |
| DOI: | 10.1007/bf02505823 |
| Popis: | Glucocorticoids, which are widely used in therapy, exert their immunosuppressive actions through specific receptors. These receptors have been characterized in cultured human skin fibroblasts and keratinocytes, but their localization in vitro and in vivo has not been established. To determine the tissue and cellular distribution of glucocorticoid receptors (GR), two specific polyclonal rabbit anti-human GR antibodies were used to detect these receptors in skin biopsy specimens, in freshly isolated and cultured human epidermal cells and in keratinocyte cell lines. Immunoreactive GR were only faintly detected in normal and abnormal differentiated cells and as well as those in the stratum granulosum and corneocytes. These immunolocalization studies were confirmed by fluorescence cell sorter analysis of isolated basal and suprabasal keratinocytes. Immunoreactive GR were highly expressed in normal cultured human keratinocytes, Langerhans cells and several cell lines whereas they were less expressed in melanocytes. Based upon these results the main targets of glucocorticoids in the epidermis appear to be basal and Langerhans cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink