Quantification of minimal residual disease in patients with e1a2 BCR-ABL-positive acute lymphoblastic leukemia using a real-time RT-PCR assay

Autor: Yukiko Tanaka, N Itou, Kazuoki Osumi, Takafumi Fukui, H Satoh, Masaru Okabe, H Hirai, Kiyoshi Kitamura, Kazuhiko Nakahara, Hiromitsu Yokota, N H Tsuno
Rok vydání: 2002
Předmět:
Adult
Male
Oncology
Cancer Research
medicine.medical_specialty
Neoplasm
Residual
Time Factors
medicine.medical_treatment
Fusion Proteins
bcr-abl
HL-60 Cells
Biology
Philadelphia chromosome
Sensitivity and Specificity
hemic and lymphatic diseases
Internal medicine
Acute lymphocytic leukemia
Tumor Cells
Cultured
medicine
Humans
RNA
Neoplasm
Aged
Bone Marrow Transplantation
Retrospective Studies
Chemotherapy
Reverse Transcriptase Polymerase Chain Reaction
Remission Induction
breakpoint cluster region
Glyceraldehyde-3-Phosphate Dehydrogenases
Reproducibility of Results
Hematology
Middle Aged
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Reference Standards
medicine.disease
Combined Modality Therapy
Minimal residual disease
Housekeeping gene
Kinetics
Treatment Outcome
Real-time polymerase chain reaction
medicine.anatomical_structure
Cancer research
Female
Bone marrow
Zdroj: Leukemia. 16:1167-1175
ISSN: 1476-5551
0887-6924
Popis: Using a real-time RT-PCR method, we analyzed the expression of e1a2 BCR-ABL mRNA in bone marrow samples from 13 patients with e1a2 BCR-ABL-positive acute lymphoblastic leukemia (ALL) at different time points during chemotherapy and after bone marrow transplantation (BMT). The detection limit of the method, assessed using serial dilutions of ALL/MIK cells, was found to be 1:10(5), similar to what is observed for the conventional RT-nested PCR method. The e1a2 BCR-ABL values were normalized with respect to those of the housekeeping gene GAPDH. The decrease in the e1a2 BCR-ABL/GAPDH ratio after remission induction chemotherapy reflects well the response to chemotherapy and consequently correlates with the prognosis. Although molecular remission was achieved by chemotherapy alone, some patients relapsed, and the e1a2 BCR-ABL/GAPDH ratios in these cases progressively increased to the levels seen prior to hematological relapse. Long-term hematological complete remission (more than 30 months) could be achieved in cases in which a more than 4.0 log decrease in the e1a2 BCR-ABL/GAPDH ratio was obtained by chemotherapy alone, and BMT was then performed. In conclusion, real-time RT-PCR allows for an evaluation of the kinetics of e1a2 BCR-ABL/GAPDH expression during the various phases of chemotherapy or after BMT and may be effective for the indication and control of disease relapse in Ph-positive ALL patients.
Databáze: OpenAIRE
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