Identification of denatured double-stranded DNA by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
| Autor: | David Horn, William H. Benner, Joe Katz, Joseph M. Jaklevic |
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| Rok vydání: | 1995 |
| Předmět: |
Matrix-assisted laser desorption electrospray ionization
Base Sequence Protein mass spectrometry Chemistry Lasers Molecular Sequence Data Organic Chemistry Oligonucleotides Analytical chemistry DNA Thermal ionization mass spectrometry Nucleic Acid Denaturation Mass spectrometry Mass Spectrometry Sample preparation in mass spectrometry Analytical Chemistry Surface-enhanced laser desorption/ionization Molecular Weight Matrix-assisted laser desorption/ionization Time-of-flight mass spectrometry Spectroscopy |
| Zdroj: | Rapid Communications in Mass Spectrometry. 9:537-540 |
| ISSN: | 1097-0231 0951-4198 |
| Popis: | We created double-stranded DNA (dsDNA) by annealing synthetic oligonucleotides and used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to measure the difference in molecular weight of the complementary strands after denaturation. Refined sample preparation and deliberate rectilinear construction at a linear mass spectrometer produced a mass resolution exceeding 1000 for single-stranded DNA. We present methodologies and demonstrate the potential for analysing dsDNA by determining the mass of the complementary strands. |
| Databáze: | OpenAIRE |
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