Evaluation of a new lymphocyte proliferation assay based on cyclic voltammetry; an alternative method
| Autor: | Mohammad Reza Nikbakht, Babak Pakbin, Gholamreza Nikbakht Brujeni |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Cell Count Lymphocyte proliferation Article 03 medical and health sciences 0302 clinical medicine Humans Lymphocytes lcsh:Science Cells Cultured Cell Proliferation Whole blood Alternative methods Multidisciplinary Chromatography Chemistry Cell growth lcsh:R In vitro toxicology Electrochemical Techniques In vitro 030104 developmental biology lcsh:Q Colorimetry Cyclic voltammetry 030217 neurology & neurosurgery Lymphocyte Proliferation Assay |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-41171-8 |
| Popis: | Lymphocyte proliferation assays are widely used to assess the cell-mediated immunity. Current in vitro testing methods that are being used have extensive applications but still more problematic, due to the technical complexity and the needs for specialized equipment and reagents. Electrochemical methods such as cyclic voltammetry represent a very promising tool for the development of label-free in vitro assays of cell proliferation and viability. Here, a novel procedure based on voltammetric behaviours of proliferating cells was fabricated. Results indicated that proliferation in cell cultures and whole blood can be monitored electrochemically using cyclic voltammetry. In the comparison with colorimetric (MTT) assay, cyclic voltammetry gave the best correlation with cell count data over a range of 1200–300,000 cells/well of a microplate. Besides the advantages of short assay duration (4 hours) and the rapidness, the possibility use of fresh blood without further processing, would give more accurate results because cells are monitoring in an intact environment. Cyclic voltammetry assay is an efficient analytical method, which can provide a simple platform for the electrochemical study of lymphocyte proliferation. |
| Databáze: | OpenAIRE |
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