Can multiple-copy sequences of prey DNA be detected amongst the gut contents of invertebrate predators?
| Autor: | Z. Jaal, Janet Hemingway, William Oliver Christian Symondson, R. H. Zaidi, Nicola J. Hawkes |
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| Rok vydání: | 1999 |
| Předmět: |
Insecticides
Food Chain Population Drug Resistance Gene Dosage Zoology Polymerase Chain Reaction law.invention Predation chemistry.chemical_compound law Pterostichus Botany Genetics Animals education Predator Gene Ecology Evolution Behavior and Systematics Polymerase chain reaction education.field_of_study biology Esterases Reproducibility of Results DNA biology.organism_classification Culex quinquefasciatus Coleoptera Culex Intestinal Absorption chemistry |
| Zdroj: | Molecular Ecology. 8:2081-2087 |
| ISSN: | 1365-294X 0962-1083 |
| DOI: | 10.1046/j.1365-294x.1999.00823.x |
| Popis: | The first experiments to clearly demonstrate that DNA techniques might be used to detect predator-prey interactions between arthropods are reported. The accurate modelling of such interactions has depended until now upon a mixture of laboratory experiments, population monitoring and biochemical tests. The latter involve gut-content analyses, and have most recently depended upon the development of prey-specific monoclonal antibodies. Although these are excellent for detecting predation on a target prey, they are impractical for analysing the prey range of a particular predator. Molecular detection depends upon the ability of DNA to resist digestion in the predator gut and of the polymerase chain reaction (PCR) to amplify prey-specific DNA from semidigested material. As a first step, experiments using carabid beetles, Pterostichus cupreus L., as predators and mosquitoes as prey are reported. The target sequences were fully characterized multiple-copy esterase genes from two laboratory strains of Culex quinquefasciatus Say. Although DNA was extracted from homogenates of whole beetles (minus appendages), a 146 bp product could be amplified from both mosquito strains digested in the beetle gut for 28 h. The larger, 263 bp product was detectable for 28 h in one mosquito strain, but could not be amplified after 5 h from the other. Whether the beetles had eaten one mosquito or six, digested for zero or 28 h, the prey were equally detectable. Having demonstrated that shorter, multiple-copy sequences survive digestion for a considerable period in the gut of a predator, the opportunity exists to develop new detection systems for studying predation in the field. |
| Databáze: | OpenAIRE |
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