A new experimental model of intrinsic denervation in ileum from wistar rats through intramural microinjections of benzalkonium chloride
| Autor: | Gabriel Luiz Montanhim, J. O Ribeiro, Andrigo Barboza De Nardi, Marília Gabriele Prado Albuquerque Ferreira, Carolina Faria Alvarenga, Isabela C.S. Marques, Sérgio Britto Garcia, Paola Castro Moraes |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Pathology
medicine.medical_specialty clinics Peritonitis Ileum short bowel syndrome benzalkonium chloride Benzalkonium chloride medicine Experimental model Myenteric plexus Denervation lcsh:Veterinary medicine General Veterinary intrinsic denervation business.industry apoptosis wistar rats medicine.disease Small intestine medicine.anatomical_structure intramural microinjections Apoptosis rodents Microinjections neurons myenterics lcsh:SF600-1100 ileum business small intestine medicine.drug myenteric plexus |
| Zdroj: | Pesquisa Veterinária Brasileira v.39 n.4 2019 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Vol 39, Iss 4, Pp 263-270 (2019) |
| Popis: | Extensive literature is available about the intrinsic denervation of segments of the digestive tube through the application of CB in the serosa of the viscera. However, this technique has some disadvantages like causing peritonitis, flanges and high mortality, limiting its use in humans. The aim of the present study was to evaluate the feasibility of benzalkonium chloride (CB) to induce intrinsic chemical denervation, through applications of CB in the intramural ileum of wistar rats, as well as deepen the knowledge about the evolution of neuronal injury caused in the process. We used 40 rats, divided into two groups (control-GC and benzalkonium-GB) of 20 animals each, divided into four sub-groups according to the time of postoperative assessment of 24, 48 hours, 30 and 90 days. The animals were submitted to intramural microinjections of sterile saline solution 0.9% (GC) or benzalkonium chloride (GB) in ileal portion, and subsequent histopathological analysis and immunohistochemistry for evaluation of neuronal injury. A significant decrease (p |
| Databáze: | OpenAIRE |
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