Detection of genetic variation among five basil species by using ISSR, SCOT and SRAP markers
| Autor: | Tasneem Y. A. Ahmed, M. H. Hussein, Shereen Abu El-Maaty, Reda E. A. Moghaieb |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International journal of health sciences. :9484-9497 |
| ISSN: | 2550-696X 2550-6978 |
| Popis: | Background: Eight inter-simple sequence repeats (ISSR), 14 Start codon-targeted (SCoT), and eleven sequence-related amplified polymorphisms were used to determine genetic diversity among five basil species (Ocimum tenuiflorum, Ocimum kilimandscharicum Guerk, Ocimum basilicum L., Ocimum americanum, and Ocimum basilicum var. minimum). Results: All of the markers' PCR products can be used to discriminate between the different basil species. The ISSR has the highest polymorphism percentage (73.1%), followed by SCoT (57.2%) and SRAP (55.6%). According to the combined data from the three markers, the basil species used were clustered into four groups, each with extremely excellent coordination between the similarity matrix and dendrograms. Species-specific markers have been identified. The SCoT analysis generates the highest number of species-specific marker (17) compared with ISSR and SRAP analysis which generated 14 and 13 markers respectively. Conclusions: These markers can be considered as a useful marker to improve Ocimum characteristics. |
| Databáze: | OpenAIRE |
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