Characterization of the Probiotic Yeast Saccharomyces boulardii in the Healthy Mucosal Immune System
| Autor: | Courtney D. McDermott, Tracey J. Lamb, Daniel Rios, Milo B. Fasken, Taryn P. Stewart, William H. Hudson, Lauren E. Hudson, Anita H. Corbett |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology lcsh:Medicine Yeast and Fungal Models Biochemistry Saccharomyces law.invention Immunoenzyme Techniques Mice Probiotic Spectrum Analysis Techniques law Immune Physiology Medicine and Health Sciences Mesenteric lymph nodes Enzyme-Linked Immunoassays lcsh:Science Immune Response Immune System Proteins Multidisciplinary biology Reverse Transcriptase Polymerase Chain Reaction Microbiota High-Throughput Nucleotide Sequencing Mucous membrane Flow Cytometry 3. Good health medicine.anatomical_structure Spectrophotometry Saccharomyces Cerevisiae Female Cytophotometry Anatomy Cellular Structures and Organelles Antibody Research Article Saccharomyces boulardii Immunology Molecular Sequence Data 030106 microbiology Context (language use) Research and Analysis Methods Real-Time Polymerase Chain Reaction Microbiology Antibodies 03 medical and health sciences Cell Walls Model Organisms Immune system medicine Animals Amino Acid Sequence RNA Messenger Immunoassays Mucous Membrane Sequence Homology Amino Acid Probiotics lcsh:R Organisms Fungi Biology and Life Sciences Proteins Cell Biology biology.organism_classification Yeast Gastrointestinal Tract Mice Inbred C57BL 030104 developmental biology Immune System Immunologic Techniques biology.protein lcsh:Q Digestive System |
| Zdroj: | PLoS ONE, Vol 11, Iss 4, p e0153351 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The probiotic yeast Saccharomyces boulardii has been shown to ameliorate disease severity in the context of many infectious and inflammatory conditions. However, use of S. boulardii as a prophylactic agent or therapeutic delivery vector would require delivery of S. boulardii to a healthy, uninflamed intestine. In contrast to inflamed mucosal tissue, the diverse microbiota, intact epithelial barrier, and fewer inflammatory immune cells within the healthy intestine may all limit the degree to which S. boulardii contacts and influences the host mucosal immune system. Understanding the nature of these interactions is crucial for application of S. boulardii as a prophylactic agent or therapeutic delivery vehicle. In this study, we explore both intrinsic and immunomodulatory properties of S. boulardii in the healthy mucosal immune system. Genomic sequencing and morphological analysis of S. boulardii reveals changes in cell wall components compared to non-probiotic S. cerevisiae that may partially account for probiotic functions of S. boulardii. Flow cytometry and immunohistochemistry demonstrate limited S. boulardii association with murine Peyer's patches. We also show that although S. boulardii induces a systemic humoral immune response, this response is small in magnitude and not directed against S. boulardii itself. RNA-seq of the draining mesenteric lymph nodes indicates that even repeated administration of S. boulardii induces few transcriptional changes in the healthy intestine. Together these data strongly suggest that interaction between S. boulardii and the mucosal immune system in the healthy intestine is limited, with important implications for future work examining S. boulardii as a prophylactic agent and therapeutic delivery vehicle. |
| Databáze: | OpenAIRE |
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