Molecular characterization and expression analysis of the interleukin 1b gene in Pacific cod (Gadus macrocephalus)
| Autor: | Yang Liu, Xia Liu, Mingkang Chen, Qianjin Cui, Mingguang Mao, Biyang Hu, Bing Chen, Chen Jiang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Fish Proteins
0301 basic medicine Interleukin-1beta Immunology Sequence alignment Transcriptome 03 medical and health sciences 0302 clinical medicine Western blot medicine Animals Gadus Gene Phylogeny Genetics biology medicine.diagnostic_test Phylogenetic tree Gene Expression Profiling Pacific cod Interleukin biology.organism_classification Immunity Innate Gadiformes Poly I-C 030104 developmental biology Sequence Alignment 030215 immunology Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 88:213-218 |
| ISSN: | 0145-305X |
| Popis: | Interleukin 1b (IL-1b) is a member of the cytokine family that serves as major mediators for early pro-inflammatory responses. During artificial breeding of Pacific cod ( Gadus macrocephalus ), outbreaks of NNV in the early development stages could lead to high mortality. Recent research reported that IL-1b participated in the host-virus interaction against NNV infection, however, no IL-1b gene has been identified from Pacific cod so far. In this study, we described the identification and characterization of a IL-1b from Pacific cod transcriptome at both transcript and protein level. Sequence alignment and phylogenetic analysis were conducted to determine and confirm their evolutionary relationship. Expression analysis revealed that IL-1b was expressed in all tested tissues and the western blot analysis confirmed the same expression profiles using purified recombinant IL-1b protein. In addition, significant up-regulation was detected in IL-1b transcripts during early development stages at 30dph and 50dph. After challenged with poly I:C, IL-1b was significantly induced after 24 h. Taken together, these results strongly suggested that IL-1b might play an essential role in innate defense mechanism of Pacific cod. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect