The fitness cost and benefit of phase-separated protein deposits

Autor: M. Madan Babu, Natalia Sanchez de Groot, Charles N. J. Ravarani, Salvador Ventura, Marc Torrent Burgas, Ala Trusina
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Medicine (General)
Natural selection
Cell
0302 clinical medicine
Phenotypic diversity
Gene expression
protein deposit
Cell fitness
Biology (General)
Quantitative Biology & Dynamical Systems
0303 health sciences
education.field_of_study
Systems Biology
Applied Mathematics
Selecció natural
natural selection
Articles
Protein Biosynthesis & Quality Control
Phenotype
3. Good health
Cell biology
Fenotip
medicine.anatomical_structure
Computational Theory and Mathematics
General Agricultural and Biological Sciences
Information Systems
QH301-705.5
Population
Phase separation
Saccharomyces cerevisiae
Biology
cell fitness
Article
General Biochemistry
Genetics and Molecular Biology
Fungal Proteins
03 medical and health sciences
R5-920
medicine
Protein deposit
Selection
Genetic
education
Fitnes
030304 developmental biology
General Immunology and Microbiology
Mechanism (biology)
Yeast
Genetic Fitness
phase separation
Proteïnes
phenotypic diversity
030217 neurology & neurosurgery
Function (biology)
Genètica
Zdroj: Recercat. Dipósit de la Recerca de Catalunya
instname
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Molecular Systems Biology
Molecular Systems Biology, Vol 15, Iss 4, Pp n/a-n/a (2019)
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Sanchez de Groot, N, Torrent Burgas, M, Ravarani, C N J, Trusina, A, Ventura, S & Babu, M M 2019, ' The fitness cost and benefit of phase-separated protein deposits ', Molecular Systems Biology, vol. 15, no. 4, e8075 . https://doi.org/10.15252/msb.20178075
DOI: 10.15252/msb.20178075
Popis: Phase separation of soluble proteins into insoluble deposits is associated with numerous diseases. However, protein deposits can also function as membrane-less compartments for many cellular processes. What are the fitness costs and benefits of forming such deposits in different conditions? Using a model protein that phase-separates into deposits, we distinguish and quantify the fitness contribution due to the loss or gain of protein function and deposit formation in yeast. The environmental condition and the cellular demand for the protein function emerge as key determinants of fitness. Protein deposit formation can influence cell-to-cell variation in free protein abundance between individuals of a cell population (i.e., gene expression noise). This results in variable manifestation of protein function and a continuous range of phenotypes in a cell population, favoring survival of some individuals in certain environments. Thus, protein deposit formation by phase separation might be a mechanism to sense protein concentration in cells and to generate phenotypic variability. The selectable phenotypic variability, previously described for prions, could be a general property of proteins that can form phase-separated assemblies and may influence cell fitness. This work was supported by the Medical Research Council (MC_U105185859; M.M.B., M.T., C.R., and N.S.G.), Marie Curie Actions (FP7-PEOPLE-2012- IEF-330352, to M.T.; and FP7-PEOPLE-2011-IEF299105, to N.S.G.), FEBS LongTerm Fellowships (N.S.G.), Beatriu de Pinos fellowships (M.T.), and the Ministerio de Economía y Competitividad (SAF2017-82158-R, SAF2015-72518-EXP, and RYC-2012-09999; M.T.). N.S.G. is a recipient of the MRC Centenary Award. M.M.B. is a Lister Institute Research Prize Fellow. We thank Gian Gaetano for supporting N.S.G. and Sean Munro for the gift of strain Y03157
Databáze: OpenAIRE
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