Impact of Hfq on the Bacillus subtilis transcriptome
| Autor: | Udo Bläsi, Ivo L. Hofacker, Branislav Večerek, Jörg Stülke, Fabian Amman, Hermann Hämmerle |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Molecular biology
lcsh:Medicine Bacillus subtilis Transcriptome Sequencing techniques RNA Small Nuclear High throughput sequencing lcsh:Science Regulation of gene expression Genetics 0303 health sciences Multidisciplinary biology Bacterial Genomics Messenger RNA Gram Positive Bacteria Microbial Genetics RNA sequencing Genomics Aerobiosis DNA-Binding Proteins RNA Bacterial RNA annealing RNA extraction Research Article DNA transcription Microbial Genomics Plasmid construction Host Factor 1 Protein Regulon Microbiology Molecular Genetics 03 medical and health sciences Bacterial Proteins DNA transciption Gene regulation Regulons Stress Physiological RNA Messenger Gene 030304 developmental biology Biology and life sciences 030306 microbiology Gene Expression Profiling lcsh:R RNA Bacteriology Gene Expression Regulation Bacterial biology.organism_classification Molecular biology techniques lcsh:Q Transcription Factors |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 6, p e98661 (2014) |
| ISSN: | 1932-6203 |
| Popis: | The RNA chaperone Hfq acts as a central player in post-transcriptional gene regulation in several Gram-negative Bacteria, whereas comparatively little is known about its role in Gram-positive Bacteria. Here, we studied the function of Hfq in Bacillus subtilis, and show that it confers a survival advantage. A comparative transcriptome analysis revealed mRNAs with a differential abundance that are governed by the ResD-ResE system required for aerobic and anaerobic respiration. Expression of resD was found to be up-regulated in the hfq- strain. Furthermore, several genes of the GerE and ComK regulons were de-regulated in the hfq- background. Surprisingly, only six out of >100 known and predicted small RNAs (sRNAs) showed altered abundance in the absence of Hfq. Moreover, Hfq positively affected the transcript abundance of genes encoding type I toxin-antitoxin systems. Taken the moderate effect on sRNA levels and mRNAs together, it seems rather unlikely that Hfq plays a central role in RNA transactions in Bacillus subtilis. peerReviewed |
| Databáze: | OpenAIRE |
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