Automated 24-hours sampling of subcutaneous tissue free cortisol in humans
| Autor: | Ragini Bhake, Stafford L. Lightman, JA Leendertz, Astrid C E Linthorst |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Microdialysis Adolescent Hydrocortisone Biomedical Engineering Enzyme-Linked Immunosorbent Assay Biology Young Adult Subcutaneous Tissue Internal medicine Abdomen medicine Humans Circadian rhythm Ultradian rhythm Sampling (statistics) General Medicine Circadian Rhythm Endocrinology medicine.anatomical_structure Free cortisol Neuroscience Hypothalamic–pituitary–adrenal axis Subcutaneous tissue Hormone |
| Zdroj: | Journal of Medical Engineering & Technology. 37:180-184 |
| ISSN: | 1464-522X 0309-1902 |
| Popis: | Hormonal systems are major regulators of metabolic and cognitive function and many of these, including the critical stress-responsive hypothalamic-pituitary-adrenal (HPA) axis, release their constituent hormones in a circadian manner. This circadian rhythmicity is made up from an underlying approximate hourly ultradian rhythm. In order to understand the importance of both circadian and ultradian rhythms in man it is important to be able to carry out multiple sampling studies over extended periods in a subject's home setting, which is the most meaningful physiological setting for homeostatically important hormones. This study has developed a novel automated sampling system that, when used in combination with a microdialysis system, collects timed samples of microdialysis fluid over a full 24 h in individuals going about their normal everyday activity. The apparatus has the capacity to provide sufficient sample volumes to measure changes in hormone concentration over 24 h, including the important period when subjects are asleep. |
| Databáze: | OpenAIRE |
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