Intracytoplasmic sperm injection: instrumentation and injection technique
| Autor: | Dianna Payne |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Cytoplasm Microinjections medicine.medical_treatment Reproductive technology Fertilization in Vitro Biology Machine learning computer.software_genre Intracytoplasmic sperm injection Endocrinology Genetics medicine Humans Instrumentation (computer programming) Molecular Biology business.industry Animal production Povidone Fetal physiology Spermatozoa Culture Media Reproductive Medicine Oocytes Animal Science and Zoology Female Artificial intelligence business Stem cell biology computer Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 7(2) |
| ISSN: | 1031-3613 |
| Popis: | A technique which is successful in one laboratory sometimes cannot be successfully applied in other laboratories, and this can be a particular problem if the technique is complex and has 'tricks' associated with it. The technique might not be fully and exhaustively detailed in a publication due to limited space in the Materials and Methods section, and yet it is often the small and seemingly unimportant points which hold the key to success. Furthermore, a specific technique might be equally successful in two laboratories which use profoundly different methodology because the same important points are applied in both and may have been arrived at independently. The trick in the application of any technique is to identify the essential components and be aware that some aspects of the technique, often enshrined in gold by a particular laboratory, may be idiosyncratic and not necessarily pivotal to its successful application. In this paper, I present a detailed description of the preparation of glass instruments for intracytoplasmic sperm injection (ICSI) and the injection technique that we use. I must stress that this is the way that ICSI has been performed successfully in our laboratory (see Payne and Matthews 1995 for clinical results) and there are subtle differences in the procedures used in other laboratories which have also successfully applied ICSI. Where possible, I outline some of these variations. |
| Databáze: | OpenAIRE |
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