Validation of the main modeling methods for the estimation of marker mean retention times in the different compartments of the gastrointestinal tract in sheep
| Autor: | E. Delval, Laurence Bernard, JP Chaise, Claude Poncet |
|---|---|
| Přispěvatelé: | Station de recherches sur la nutrition des herbivores, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Rok vydání: | 1998 |
| Předmět: |
Male
030309 nutrition & dietetics Biology Abomasum Models Biological 03 medical and health sciences Rumen Random Allocation Animal science Reticulorumen Latin square Digestive System Physiological Phenomena Genetics medicine Animals [SDV.SA.SPA] Life Sciences [q-bio]/Agricultural sciences/Animal production studies Ytterbium 10. No inequality ComputingMilieux_MISCELLANEOUS Radioisotopes 0303 health sciences Sheep Stomach 0402 animal and dairy science Technetium 04 agricultural and veterinary sciences General Medicine Anatomy 040201 dairy & animal science Chromium Radioisotopes medicine.anatomical_structure [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies Isotope Labeling Hay Animal Science and Zoology Digestion Texel Food Science |
| Zdroj: | HAL Journal of Animal Science Journal of Animal Science, American Society of Animal Science, 1998, 76 (9), pp.2485-2495 |
| ISSN: | 0021-8812 1525-3163 |
| Popis: | Four Texel wethers (60 to 64 kg) fitted with rumen and duodenal cannulae were used to compare methods for estimating mean retention times (MRT) of digesta markers. They were fed, in eight equal meals, 1,200 g of a mixture of a chopped and ground (8-mm screen) and pelleted orchardgrass hay in the ratios 90/10, 50/50, 30/70, or 10/90 according to a 4 x 4 Latin square design. Mean retention time in the stomach and in the whole tract were estimated by orally delivering pulse doses of 170 Tm-labeled chopped hay, 169 Yb-labeled ground hay, and [ 51 Cr]EDTA, followed by duodenal spot-sampling and total collection of feces; calculations were done using an algebraic method. Mean retention time in the abomasum was estimated following administration of a pulse dose of the markers ( 169 Yb-labeled duodenal particles and [ 51 Cr]EDTA) in the abomasum via the ruminal cannula through the reticulo-omasal orifice and collecting duodenal samples. The reference value for MRT in the reticulorumen (MRT RR ) was calculated by subtracting MRT in the abomasum from MRT in the stomach. For all markers, fecal excretion curves were fitted to a two-compartment age-independent model, a gamma-2 age dependent-age independent two-compartmental model, and a multicompartmental model. Comparisons were made among parameters of the models and their anatomical or physiological attributes when these were clearly defined or easily calculated. The mean retention time in the reticulorumen (MRT RR ) obtained from the multicompartment model was similar to those calculated with the algebraic method whatever the sampling site and the marker used. |
| Databáze: | OpenAIRE |
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