IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria

Autor: Piyatida Tangteerawatana, Srisin Khusmith, Thareerat Kalambaheti, Masashi Hayano, Hedvig Perlmann, Marita Troye-Blomberg
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Malaria Journal, Vol 8, Iss 1, p 286 (2009)
Malaria Journal
ISSN: 1475-2875
Popis: BackgroundTheIL4-590 gene polymorphism has been shown to be associated with elevated levels of anti-Plasmodium falciparumIgG antibodies and parasite intensity in the malaria protected Fulani of West Africa. This study aimed to investigate the possible impact ofIL4-590C/T polymorphism on anti-P. falciparumIgG subclasses and IgE antibodies levels and the alteration of malaria severity in complicated and uncomplicated malaria patients with or without previous malaria experiences.MethodsAnti-P.falciparumIgG subclasses and IgE antibodies in plasma of complicated and uncomplicated malaria patients with or without previous malaria experiences were analysed using ELISA.IL4-590 polymorphisms were genotyped using RFLP-PCR. Statistical analyses of the IgG subclass levels were done by Oneway ANOVA. Genotype differences were tested by Chi-squared test.ResultsTheIL4-590T allele was significantly associated with anti-P. falciparumIgG3 antibody levels in patients with complicated (P= 0.031), but not with uncomplicated malaria (P= 0.622). Complicated malaria patients with previous malaria experiences carryingIL4-590TT genotype had significantly lower levels of anti-P. falciparumIgG3 (P= 0.0156), while uncomplicated malaria patients with previous malaria experiences carrying the same genotype had significantly higher levels(P= 0.0206) compared to theirIL4-590 counterparts. The different anti-P. falciparumIgG1 and IgG3 levels among IL4 genotypes were observed. Complicated malaria patients with previous malaria experiences tended to have lower IgG3 levels in individuals carrying TT when compared to CT genotypes (P= 0.075). In contrast, complicated malaria patients without previous malaria experiences carrying CC genotype had significantly higher anti-P. falciparumIgG1 than those carrying either CT or TT genotypes (P= 0.004,P= 0.002, respectively).ConclusionThe results suggest thatIL4-590C or T alleles participated differently in the regulation of anti-malarial antibody isotype profiles in primary and secondary malaria infection and, therefore, could play an important role in alteration of malaria severity.
Databáze: OpenAIRE
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