A prime-boost vaccination of mice with attenuated Salmonella expressing a 30-mer peptide from the Trichinella spiralis gp43 antigen
| Autor: | Fernando Ruiz-Perez, Rocío Fonseca-Liñán, Nicolás Villegas-Sepúlveda, Guadalupe Ortega-Pierres, A.M. Castillo Alvarez, A. Vaquero-Vera |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Salmonella
Recombinant Fusion Proteins Trichinella spiralis Antibodies Helminth Gene Expression Trichinella Helminth genetics medicine.disease_cause Microbiology Mice Antigen Antigens Neoplasm parasitic diseases medicine Animals Mice Inbred BALB C General Veterinary biology Muscles Vaccination Trichinellosis General Medicine biology.organism_classification Virology Intestines Immunization Salmonella enterica Antigens Helminth Larva Female Parasitology |
| Zdroj: | Veterinary Parasitology. 194:202-206 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2013.01.056 |
| Popis: | Protection against Trichinella infections has been achieved using various parasite antigens and adjuvants. Recently, we reported that immunization of mice with an attenuated Salmonella strain displaying a 30-mer peptide (residues 210-239) from the Trichinella spiralis gp43 antigen using the ShdA autotransporter induced partial protection against T. spiralis infection. To improve the efficacy of vaccination, we used the MisL autotransporter system to display the Ts30mer peptide on the surface of Salmonella enterica ser. Typhimurium in combination with a prime-boost vaccination strategy. This vector and immunization regimen induced superior protection against T. spiralis when compared to our previously reported approach. Data presented herein showed a significant reduction in adult worm and muscle larvae burdens, high IgG titers, and increased production of intestinal mucus with entrapped adult worms. This prime-boost vaccination scheme is a suitable strategy to elicit enhanced protective immunity against T. spiralis. |
| Databáze: | OpenAIRE |
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