Telomerase activity in human leukemic cell lines is inhibited by antisense pentadecadeoxynucleotides targeted against c-myc mRNA
| Autor: | Morinobu Takahashi, Kohtaro Fujimoto |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Telomerase
Biophysics HL-60 Cells Biology Biochemistry Proto-Oncogene Mas Proto-Oncogene Proteins c-myc Telomerase RNA component Transforming Growth Factor beta Sense (molecular biology) Tumor Cells Cultured Humans Telomerase reverse transcriptase RNA Messenger Molecular Biology Cell Line Transformed Oligonucleotide Tumor Necrosis Factor-alpha Cell Biology Cell cycle Oligonucleotides Antisense Molecular biology Cell biology Enzyme Activation Oligodeoxyribonucleotides Cell culture Leukemia Erythroblastic Acute K562 cells |
| Zdroj: | Biochemical and biophysical research communications. 241(3) |
| ISSN: | 0006-291X |
| Popis: | Telomerase is a ribonucleoprotein complex that is thought to add telomeric repeats to the ends of chromosomes during the replicative phase of the cell cycle. We tested the hypothesis that proto-oncogene c-myc plays an essential role in the regulation of telomerase activity in vertebrate cells by exposing three human leukemic cell lines, HL60, U937, and K562, to 15-mer antisense c-myc oligonucleotides. All the treated cells showed a profound decrease in telomerase activity after c-myc antisense oligomer treatment, whereas cells treated with c-myc sense oligomers showed essentially no change in telomerase activity. |
| Databáze: | OpenAIRE |
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