Vellozia flavicans Mart. ex Schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by Bothrops jararacussu venom

Autor: Francisco Carlos Groppo, Karina Cogo, Miriéle Cristina Ferraz, Ana Paula Guerreiro Bentes, Alexandro Mateus da Silva, Yoko Oshima-Franco, José Carlos Cogo, Magali Glauzer Silva, Márcio Galdino dos Santos, Eliana Aparecida Varanda, Natália Tribuiani, Talita Signoreti Graziano
Přispěvatelé: Univ Sorocaba UNISO, Universidade Estadual de Campinas (UNICAMP), Tocantins Fed Univ UFT, Serpentarium Vale Paraiba Univ CEN UNIVAP, Universidade Estadual Paulista (Unesp), de Santo Amaro Univ UNISA
Rok vydání: 2013
Předmět:
Zdroj: BMC Complementary and Alternative Medicine
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1472-6882
Popis: Made available in DSpace on 2014-12-03T13:09:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-02-08Bitstream added on 2014-12-03T13:23:14Z : No. of bitstreams: 1 WOS000331126400001.pdf: 1054034 bytes, checksum: 5df0011a0e68dba2f72cb7f944e14d37 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Snakebite is a significant public health issue in tropical countries. In Brazil, some of the most common snake envenomations are from Bothrops. Bothrops bites trigger local and systemic effects including edema, pain, erythema, cyanosis, infections, and necrosis. Vellozia flavicans is a plant from the Brazilian cerrado (savanna) that is popularly used as an anti-inflammatory medicine. Since inflammation develops quickly after Bothrops bites, which can lead to infection, the aim of the present study was to observe possible anti-snake venom and antimicrobial activities of V. flavicans (Vf).Methods: The chromatographic profile of the main constituents from the Vf leaf hydroalcoholic extract was obtained by thin-layer chromatography (TLC). The anti-snake venom activity was measured by Vf's ability to neutralize the in vitro neuromuscular blockade caused by Bothrops jararacussu venom (Bjssu) in a mouse phrenic nerve-diaphragm model (PND). After a 20 min incubation, preparations of PND were added to Tyrode's solution (control); Vf (0.2, 0.5, 1, and 2 mg/mL); 40 mu g/mL Bjssu; pre-incubation for 30 min with Bjssu and 1 mg/mL Vf; and a Bjssu pretreated preparation (for 10 min) followed by 1 mg/mL Vf. Myographic recording was performed, and the contractile responses were recorded. The antimicrobial activity (minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC]) was obtained for Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis, using gentamicin and vancomycin as positive controls.Results: TLC analysis yielded several compounds from Vf, such as flavonoids (quercetin) and phenolic acids (chlorogenic acid). Bjssu completely blocked the contractile responses of PND preparations, while Vf preserved 97% (+/- 10%) of the contractile responses when incubated with Bjssu. In the PND pretreated with Bjssu, Vf was able to inhibit the neuromuscular blockade progress. MIC and MBC of Vf ranged from 2.5 to 5.0 mg/mL for P. aeruginosa and S. aureus strains, while no antimicrobial activity was observed for E. coli and E. faecalis.Conclusions: The hydroalcoholic extract from Vf leaves was able to neutralize and decrease the in vitro neuromuscular blockade caused by Bjssu. However, it did not show significant antimicrobial activity against the tested bacteria. Univ Sorocaba UNISO, Pharmaceut Sci Postgrad Program, BR-18023000 Sorocaba, SP, Brazil Univ Campinas UNICAMP, Piracicaba Dent Sch, BR-13414903 Piracicaba, SP, Brazil Tocantins Fed Univ UFT, Environm Sci Postgrad Program, PGCiamb, BR-77001090 Porto Nacl, Tocantins, Brazil Serpentarium Vale Paraiba Univ CEN UNIVAP, BR-12244000 Sao Jose Dos Campos, SP, Brazil Sao Paulo State Univ UNESP, Pharmaceut Sci Fac Araraquara, BR-14801902 Sao Paulo, Brazil de Santo Amaro Univ UNISA, Dept Dent, BR-04829300 Santo Amaro, SP, Brazil Sao Paulo State Univ UNESP, Pharmaceut Sci Fac Araraquara, BR-14801902 Sao Paulo, Brazil FAPESP: 04/09705-8 FAPESP: 07/53883-6 FAPESP: 08/52643-4 FAPESP: 08/11005-5 FAPESP: 12/08271-0
Databáze: OpenAIRE
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