Effectiveness of antimicrobial peptide immobilization for preventing perioperative cornea implant-associated bacterial infection
| Autor: | Shouping Liu, Melina Setiawan, Donald T.H. Tan, Rajamani Lakshminarayanan, Chan Lwin Nyein, Padmanabhan Saraswathi, Jodhbir S. Mehta, Xiao Wei Tan, Tze Wei Goh, Roger W. Beuerman, Andri K. Riau |
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| Rok vydání: | 2014 |
| Předmět: |
Corneal Infection
genetic structures medicine.disease_cause Keratitis Microbiology Corneal Transplantation In vivo Cornea medicine Animals Surgical Wound Infection Pharmacology (medical) Pseudomonas Infections Experimental Therapeutics Pharmacology Titanium Slit Lamp Chemistry Pseudomonas aeruginosa Staphylococcal Infections medicine.disease Antimicrobial eye diseases Anti-Bacterial Agents Infectious Diseases medicine.anatomical_structure Staphylococcus aureus Implant sense organs Rabbits Tomography Optical Coherence Antimicrobial Cationic Peptides |
| Zdroj: | Antimicrobial agents and chemotherapy. 58(9) |
| ISSN: | 1098-6596 |
| Popis: | Titanium (Ti) is a promising candidate biomaterial for an artificial corneal skirt. Antimicrobial peptide (AMP) immobilization may improve the bactericidal effect of the Ti substrate. In this study, we tested the bactericidal efficacy of a functionalized Ti surface in a rabbit keratitis model. A corneal stromal pocket was created by a femtosecond laser. The Ti films were then inserted into the pocket, and Staphylococcus aureus or Pseudomonas aeruginosa was inoculated into the pocket above the implant films. The corneas with Ti-AMP implants were compared with the corneas implanted with unprotected Ti by slit lamp observation and anterior segment optical coherence tomography (AS-OCT). Inflammatory responses were evaluated by bacterium counting, hematoxylin-eosin staining, and immunostaining. There was a lower incidence and a lesser extent of infection on rabbit corneas with Ti-AMP implants than on those with unprotected Ti implants. The bactericidal effect of AMP against S. aureus was comparable to that of postoperative prophylactic antibiotic treatment; hence, SESB2V AMP bound to the Ti implant provided functional activity in vivo , but its efficacy was greater against S. aureus than against P. aeruginosa . This work suggests that SESB2V AMP can be successfully functionalized in a rabbit keratitis model to prevent perioperative corneal infection. |
| Databáze: | OpenAIRE |
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