A 74-bp Promoter of the Tnp2 Gene Confers Testis- and Spermatid-Specific Expression in Transgenic Mice
| Autor: | Özlem Topaloglu, Wolfgang Engel, Karim Nayernia, Gregor Schlüter |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Male Untranslated region Time Factors Spermiogenesis Molecular Sequence Data Biophysics Mice Transgenic Biology Biochemistry Primer extension Mice 03 medical and health sciences 0302 clinical medicine Start codon Transcription (biology) Testis Gene expression Cyclic AMP medicine Animals Tissue Distribution RNA Messenger Transgenes Promoter Regions Genetic 3' Untranslated Regions Molecular Biology Gene DNA Primers 030304 developmental biology 0303 health sciences Base Sequence Spermatid urogenital system Homozygote Nuclear Proteins Cell Biology Blotting Northern Spermatids Spermatozoa Molecular biology Rats DNA-Binding Proteins Germ Cells medicine.anatomical_structure 030220 oncology & carcinogenesis RNA 5' Untranslated Regions Plasmids |
| Zdroj: | Biochemical and Biophysical Research Communications. 289:597-601 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2001.5999 |
| Popis: | During the final stages of spermatogenesis, round spermatids undergo several morphological, biochemical, and physiological modifications which result in the formation of mature spermatozoa. One of these is the nuclear condensation, achieved by the replacement of somatic-type and testis-specific histones by transition proteins and protamines leading to cessation of transcription several days before the completion of spermiogenesis. Therefore, a strict temporal and stage-specific gene expression is necessary for the correct differentiation of round spermatids into mature spermatozoa. In this study, the 5' regulatory region of rat Tnp2 gene was investigated by primer extension analysis and transgenic mice study. Primer extension analysis revealed a transcription start site which lies 70 bp upstream of the translation start codon. By transgenic mice studies, we demonstrated that a 147-bp 5' untranslated region corresponding to the region -74 to +73 is sufficient to confer testis- and spermatid-specific expression of rat Tnp2 gene. |
| Databáze: | OpenAIRE |
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