A Facile Method for the Non-Covalent Amine Functionalization of Carbon-Based Surfaces for Use in Biosensor Development
| Autor: | Jon E. Evans, Zari Tehrani, Jacob John Mitchell, S. Rozhko, Muhammad Munem Ali, Ryan Bigham, Owen J. Guy, Olga Kazakova, Ehsaneh Daghigh Ahmadi, Ffion Walters, Anitha Devadoss, Hina Y. Abbasi, Gregory Burwell |
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| Jazyk: | angličtina |
| Předmět: |
Materials science
General Chemical Engineering real-time Nanotechnology 02 engineering and technology 010402 general chemistry biosensor 01 natural sciences Article law.invention lcsh:Chemistry symbols.namesake resistor X-ray photoelectron spectroscopy law sensor Molecule General Materials Science Nanocomposite nanocomposite Graphene GFET graphene non-covalent 021001 nanoscience & nanotechnology 0104 chemical sciences lcsh:QD1-999 symbols Surface modification Amine gas treating drop-cast 0210 nano-technology Raman spectroscopy carbon-surfaces Biosensor π-π stacking |
| Zdroj: | Nanomaterials Volume 10 Issue 9 Nanomaterials, Vol 10, Iss 1808, p 1808 (2020) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano10091808 |
| Popis: | Affinity biosensors based on graphene field-effect transistor (GFET) or resistor designs require the utilization of graphene&rsquo s exceptional electrical properties. Therefore, it is critical when designing these sensors, that the electrical properties of graphene are maintained throughout the functionalization process. To that end, non-covalent functionalization may be preferred over covalent modification. Drop-cast 1,5-diaminonaphthalene (DAN) was investigated as a quick and simple method for the non-covalent amine functionalization of carbon-based surfaces such as graphene, for use in biosensor development. In this work, multiple graphene surfaces were functionalized with DAN via a drop-cast method, leading to amine moieties, available for subsequent attachment to receptor molecules. Successful modification of graphene with DAN via a drop-cast method was confirmed using X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and real-time resistance measurements. Successful attachment of receptor molecules also confirmed using the aforementioned techniques. Furthermore, an investigation into the effect of sequential wash steps which are required in biosensor manufacture, on the presence of the DAN layer, confirmed that the functional layer was not removed, even after multiple solvent exposures. Drop-cast DAN is thus, a viable fast and robust method for the amine functionalization of graphene surfaces for use in biosensor development. |
| Databáze: | OpenAIRE |
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