LncRNA H19 interacted with miR‐130a‐3p and miR‐17‐5p to modify radio‐resistance and chemo‐sensitivity of cardiac carcinoma cells

Autor: Xinxin Zhang, Jing Li, Zhixiang Li, Jianguang Jia, Hongbo Li, Dankai Zhan, Jun Qian
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Cancer Research
medicine.medical_treatment
Apoptosis
Radiation Tolerance
Heart Neoplasms
Mice
0302 clinical medicine
Medicine
Original Research
Cancer Biology
cardiac cancer
radio‐sensitivity
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
lncRNA H19
Oncology
chemo‐sensitivity
030220 oncology & carcinogenesis
Female
RNA
Long Noncoding

medicine.drug
Adult
miR‐130a‐3p
Antineoplastic Agents
lcsh:RC254-282
03 medical and health sciences
miR‐17‐5p
Cell Line
Tumor

Carcinoma
Animals
Humans
Radiology
Nuclear Medicine and imaging

Viability assay
IC50
Survival rate
cell viability
Aged
Cell Proliferation
Neoplasm Staging
Cisplatin
cell apoptosis
business.industry
Mir 17 5p
medicine.disease
Radiation therapy
Disease Models
Animal

MicroRNAs
030104 developmental biology
Drug Resistance
Neoplasm

Cancer research
Neoplasm Grading
business
Zdroj: Cancer Medicine
Cancer Medicine, Vol 8, Iss 4, Pp 1604-1618 (2019)
ISSN: 2045-7634
DOI: 10.1002/cam4.1860
Popis: The current investigation explored the synthetic contribution of lncRNA H19, miR‐130a‐3p, and miR‐17‐5p to radio‐resistance and chemo‐sensitivity of cardiac cancer cells. Totally 284 human cardiac cancer tissues were gathered, and they have been pathologically diagnosed. The cardiac cancer cells were isolated with utilization of the mechanic method. Moreover, cisplatin, adriamycin, mitomycin, and 5‐fluorouracil were designated as the chemotherapies, and single‐dose X‐rays were managed as the radiotherapy for cardiac cancer cells. We also performed luciferase reporter gene assay to verify the targeted relationship between H19 and miR‐130a‐3p, as well as between H19 and miR‐17‐5p. Finally, mice models were established to examine the functions of H19, miR‐130a‐3p, and miR‐17‐5p on the development of cardiac cancer. The study results indicated that H19, miR‐130a‐3p, and miR‐17‐5p expressions within cardiac cancer tissues were significantly beyond those within adjacent nontumor tissues (P
Databáze: OpenAIRE
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