LncRNA H19 interacted with miR‐130a‐3p and miR‐17‐5p to modify radio‐resistance and chemo‐sensitivity of cardiac carcinoma cells
Autor: | Xinxin Zhang, Jing Li, Zhixiang Li, Jianguang Jia, Hongbo Li, Dankai Zhan, Jun Qian |
---|---|
Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine Cancer Research medicine.medical_treatment Apoptosis Radiation Tolerance Heart Neoplasms Mice 0302 clinical medicine Medicine Original Research Cancer Biology cardiac cancer radio‐sensitivity Middle Aged lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens lncRNA H19 Oncology chemo‐sensitivity 030220 oncology & carcinogenesis Female RNA Long Noncoding medicine.drug Adult miR‐130a‐3p Antineoplastic Agents lcsh:RC254-282 03 medical and health sciences miR‐17‐5p Cell Line Tumor Carcinoma Animals Humans Radiology Nuclear Medicine and imaging Viability assay IC50 Survival rate cell viability Aged Cell Proliferation Neoplasm Staging Cisplatin cell apoptosis business.industry Mir 17 5p medicine.disease Radiation therapy Disease Models Animal MicroRNAs 030104 developmental biology Drug Resistance Neoplasm Cancer research Neoplasm Grading business |
Zdroj: | Cancer Medicine Cancer Medicine, Vol 8, Iss 4, Pp 1604-1618 (2019) |
ISSN: | 2045-7634 |
DOI: | 10.1002/cam4.1860 |
Popis: | The current investigation explored the synthetic contribution of lncRNA H19, miR‐130a‐3p, and miR‐17‐5p to radio‐resistance and chemo‐sensitivity of cardiac cancer cells. Totally 284 human cardiac cancer tissues were gathered, and they have been pathologically diagnosed. The cardiac cancer cells were isolated with utilization of the mechanic method. Moreover, cisplatin, adriamycin, mitomycin, and 5‐fluorouracil were designated as the chemotherapies, and single‐dose X‐rays were managed as the radiotherapy for cardiac cancer cells. We also performed luciferase reporter gene assay to verify the targeted relationship between H19 and miR‐130a‐3p, as well as between H19 and miR‐17‐5p. Finally, mice models were established to examine the functions of H19, miR‐130a‐3p, and miR‐17‐5p on the development of cardiac cancer. The study results indicated that H19, miR‐130a‐3p, and miR‐17‐5p expressions within cardiac cancer tissues were significantly beyond those within adjacent nontumor tissues (P |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |