The Cytotoxic Effect of Apis mellifera Venom with a Synergistic Potential of Its Two Main Components—Melittin and PLA2—On Colon Cancer HCT116 Cell Lines

Autor: Ziad Fajloun, Bruno Coutard, Dany El-Obeid, Jean-Marc Sabatier, Mariam Rifi, Carole Yaacoub, Hiba Mawlawi
Přispěvatelé: Institut de neurophysiopathologie (INP), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2021
Předmět:
MESH: Cell Death
Pharmaceutical Science
Venom
Pharmacology
MESH: Drug Synergism
MESH: Bees
Analytical Chemistry
chemistry.chemical_compound
0302 clinical medicine
hemic and lymphatic diseases
Drug Discovery
Cytotoxic T cell
MESH: Animals
Cytotoxicity
MESH: Phospholipases A2
Chromatography
High Pressure Liquid
0303 health sciences
Cell Death
biology
Chemistry
Drug Synergism
Bees
bee venom
3. Good health
MESH: Cell Survival
MESH: Melitten
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Colonic Neoplasms
Molecular Medicine
lipids (amino acids
peptides
and proteins)
Apis mellifera
MESH: HCT116 Cells
Cell Survival
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Article
in vitro anticancer effect
Melittin
lcsh:QD241-441
03 medical and health sciences
Phospholipase A2
lcsh:Organic chemistry
Animals
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Viability assay
Physical and Theoretical Chemistry
MESH: Chromatography
High Pressure Liquid
neoplasms
030304 developmental biology
MESH: Colonic Neoplasms
PLA2
MESH: Humans
HCT116 cell lines
Organic Chemistry
HCT116 Cells
Melitten
In vitro
Phospholipases A2
melittin
Cancer cell
biology.protein
Zdroj: Molecules
Volume 26
Issue 8
Molecules, MDPI, 2021, 26 (8), pp.2264. ⟨10.3390/molecules26082264⟩
Molecules, Vol 26, Iss 2264, p 2264 (2021)
Molecules, 2021, 26 (8), pp.2264. ⟨10.3390/molecules26082264⟩
ISSN: 1420-3049
DOI: 10.3390/molecules26082264
Popis: Colon carcinogenesis is ranked second globally among human diseases after cardiovascular failures. Bee venom (BV) has been shown to possess in vitro anticancer effects against several types of cancer cells. The two main biopeptides of Apis mellifera BV, namely, melittin (MEL) and phospholipase A2 (PLA2), are suspected to be the biomolecules responsible for the anticancer activity. The present work aims to evaluate the cytotoxic effect of the A. mellifera venom on human colon carcinoma cells (HCT116), and to assess the synergistic effect of MEL and PLA2 on these cells. After analyzing, through high-pressure liquid chromatography, the proportions of MEL and PLA2 on BV, we have established a cell viability assay to evaluate the effect of BV, MEL, PLA2, and a mixture of MEL and PLA2 on the HCT116 cells. Results obtained showed a strong cytotoxicity effect induced by the A. mellifera venom and to a lower extent MEL or PLA2 alone. Remarkably, when MEL and PLA2 were added together, their cytotoxic effect was greatly improved, suggesting a synergistic activity on HCT116 cells. These findings confirm the cytotoxic effect of the A. mellifera venom and highlight the presence of synergistic potential activities between MEL and PLA2, possibly inducing membrane disruption of HCT116 cancer cells. Altogether, these results could serve as a basis for the development of new anticancer treatments.
Databáze: OpenAIRE
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