Cyclic stretch reveals a mechanical role for intermediate filaments in a desminopathic cell model
| Autor: | E. Leccia, Florence Delort, Véronique Pizon, F. Briki, V Bailleux, M Pelloux, Jean Doucet, Sabrina Batonnet-Pichon, Patrick Vicart, A. Tarze |
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| Rok vydání: | 2012 |
| Předmět: |
Cell
Intermediate Filaments Biophysics Context (language use) macromolecular substances Biology Desmin Myoblasts Muscular Diseases Structural Biology Microtubule Cell Adhesion medicine Humans Myocyte Intermediate filament Molecular Biology Cells Cultured Actin Cell Biology Anatomy musculoskeletal system Actin cytoskeleton medicine.anatomical_structure Mutation Stress Mechanical |
| Zdroj: | Physical Biology. 10:016001 |
| ISSN: | 1478-3975 1478-3967 |
| DOI: | 10.1088/1478-3975/10/1/016001 |
| Popis: | Mechanics is now recognized as crucial in cell function. To date, the mechanical properties of cells have been inferred from experiments which investigate the roles of actin and microtubules ignoring the intermediate filaments (IFs) contribution. Here, we analyse myoblasts behaviour in the context of myofibrillar myopathy resulting from p.D399Y desmin mutation which disorganizes the desmin IF network in muscle cells. We compare the response of myoblasts expressing either mutated or wild-type desmin to cyclic stretch. Cells are cultivated on supports submitted to periodic uniaxial stretch of 20% elongation amplitude and 0.3 Hz frequency. We show that during stretching cycles, cells expressing mutated desmin reduce their mean amplitude both for the elongation and spreading area compared to those expressing wild-type desmin. Even more unexpected, the reorientation angles are altered in the presence of p.D399Y desmin. Yet, at rest, the whole set of those parameters are similar for the two cell populations. Thus, we demonstrate that IFs affect the mechanical properties and the dynamics of cell reorientation. Since these processes are known due to actin cytoskeleton, these results suggest the IFs implication in mechanics signal transduction. Further studies may lead to better understanding of their contribution to this process. |
| Databáze: | OpenAIRE |
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