Determination of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry

Autor: Zhi-Ling Zhou, Xiaoping Fan, Jian Zhuang, Ping Zhu, Huiming Guo, Cong Lu, Xue-jun Xiao, Shao-yi Zheng, Jimei Chen, Yu-Guan Wen, Ruixin Fan, Ruobin Wu
Rok vydání: 2011
Předmět:
Zdroj: Journal of Pharmaceutical and Biomedical Analysis. 54:198-202
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2010.07.016
Popis: A sensitive and rapid method was developed for quantification of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry (LC–MS/MS). An aliquot of 1 mL plasma sample was extracted with ethyl acetate–dichloromethane. Separation of olprinone and the milrinone (internal standard, IS) from the interferences was achieved on a C18 column followed by MS/MS detection. The analytes were monitored in the positive ionization mode. Multiple reaction monitoring using the transition of m/z 251 → m/z 155 and m/z 212 → m/z 140 was performed to quantify olprinone and IS, respectively. The method had a total chromatographic run time of 3 min and linear calibration curves over the concentration range of 0.5–60 ng/mL. The lower limit of quantification (LLOQ) was 0.5 ng/mL. The intra- and inter-day precisions were less than 16.3% for low QC level, and 7.1% for other QC levels, respectively. The intra- and inter-day relative errors were ranged between −12.2% and 3.7% for three QC concentration levels. The validated method was successfully applied to the quantification of olprinone concentration in human plasma after intravenous (i.v.) administration of olprinone at a constant rate of infusion of 2 μg/(kg min) for 5 min in order to evaluate the pharmacokinetics.
Databáze: OpenAIRE
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