Effect of sealing infected dentin with glass ionomer cement on the abundance and localization of MMP-2, MMP-8, and MMP-9 in young permanent molarsin vivo
| Autor: | Denise Stadler Wambier, M.R. Carrilho, Eunice Kuhn, Eduardo Bauml Campagnoli, Alessandra Reis, Ana Cláudia Rodrigues Chibinski |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Molar Adolescent 0206 medical engineering Glass ionomer cement Dentistry 02 engineering and technology Dental Caries Matrix (biology) Matrix metalloproteinase Composite Resins Immunoenzyme Techniques 03 medical and health sciences 0302 clinical medicine stomatognathic system In vivo Dentin medicine Humans Child General Dentistry business.industry 030206 dentistry 020601 biomedical engineering Dentition Permanent stomatognathic diseases Matrix Metalloproteinase 8 medicine.anatomical_structure Matrix Metalloproteinase 9 Glass Ionomer Cements Matrix Metalloproteinase 2 Immunohistochemistry Female business Brazil Immunostaining |
| Zdroj: | International Journal of Paediatric Dentistry. 26:125-133 |
| ISSN: | 0960-7439 |
| DOI: | 10.1111/ipd.12167 |
| Popis: | Background The study of MMPs’ behavior in carious lesions contributes to the understanding of the mechanisms involved in dentin reorganization after restoration. Aim To compare the abundance and localization of MMPs 2, 8, and 9 in infected dentin before and after restoration. Design The sample consisted of 23 young permanent molars with active deep carious lesions. Infected carious dentin samples were collected from the same tooth at baseline and 60 days after cavity lining with GIC and composite resin restoration and processed for immunohistochemistry assays. After digital images were obtained, two calibrated operators analyzed the samples according to the immunostaining intensity and the MMPs’ localization. Chi-square test was used for statistical analysis. Results The intensity of immunostaining for MMP-8 was reduced after 60 days (P = 0.02), and no difference was observed for MMP-2 (P = 0.32) and MMP-9 (P = 0.14). The MMPs’ distribution was generalized in the intertubular dentin and absent or located in the intratubular dentin, regardless of the period. Conclusion The sealing of infected carious dentin in young permanent molars reduced the expression of MMP-8, which is consistent with the initial remodeling process of the dentin matrix. |
| Databáze: | OpenAIRE |
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