CD248 enhances tissue factor procoagulant function, promoting arterial and venous thrombosis in mouse models
| Autor: | Alan E. Mast, Scott C. Meixner, Wolfram Ruf, Piyushkumar R. Kapopara, Victor Lei, Edward M. Conway, Kevin Gonzalez, Houra Loghmani, Edward L.G. Pryzdial, Jenny Li-Ying Huang, Nooshin Safikhan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Inflammation
Factor VIIa 030204 cardiovascular system & hematology Inferior vena cava Article Thromboplastin 03 medical and health sciences Tissue factor chemistry.chemical_compound Mice 0302 clinical medicine Thrombin Tissue factor pathway inhibitor Antigens CD Antigens Neoplasm medicine Animals Humans Mice Knockout Venous Thrombosis medicine.diagnostic_test Factor X Hematology Coagulation chemistry medicine.vein Cancer research Prothrombin Time medicine.symptom medicine.drug Partial thromboplastin time |
| Zdroj: | J Thromb Haemost |
| Popis: | BACKGROUND: CD248 is a pro-inflammatory, transmembrane glycoprotein expressed by vascular smooth muscle cells (VSMC), monocytes/macrophages, and other cells of mesenchymal origin. Its distribution and properties are reminiscent of those of the initiator of coagulation, tissue factor (TF). OBJECTIVE: We examined whether CD248 also participates in thrombosis. METHODS: We evaluated the role of CD248 in coagulation using mouse models of vascular injury, and by assessing its functional interaction with the TF-factor VIIa (FVIIa)-factor X (FX) complex. RESULTS: The time to ferric chloride-induced occlusion of the carotid artery in CD248 knockout (KO) mice was significantly longer than in wild-type (WT) mice. In an inferior vena cava (IVC) stenosis model of thrombosis, lack of CD248 conferred relative resistance to thrombus formation compared to WT mice. Levels of circulating cells and coagulation factors, prothrombin time, activated partial thromboplastin time, and tail bleeding times were similar in both groups. Proximity ligation assays revealed that TF and CD248 are |
| Databáze: | OpenAIRE |
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