The nuclear transcription factor, TAF7, is a cytoplasmic regulator of protein synthesis
| Autor: | Dan Cheng, Kevin Semmens, Elizabeth McManus, Qingrong Chen, Daoud Meerzaman, Xiantao Wang, Markus Hafner, Brian A. Lewis, Hidehisa Takahashi, Ballachanda N. Devaiah, Anne Gegonne, Dinah S. Singer |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| Popis: | Description Transcription factor TAF7 links transcription and translation by delivering its transcripts to polysomes for translation. The TFIID component, TAF7, has been extensively characterized as essential for transcription and is critical for cell proliferation and differentiation. Here, we report that TAF7 is a previously unknown RNA chaperone that contributes to the regulation of protein synthesis. Mechanistically, TAF7 binds RNAs in the nucleus and delivers them to cytoplasmic polysomes. A broad spectrum of target RNA species, including the HIV-1 transactivation response element, binds TAF7 through consensus CUG motifs within the 3′ untranslated region. Export to the cytoplasm depends on a TAF7 nuclear export signal and occurs by an exportin 1–dependent pathway. Notably, disrupting either TAF7’s RNA binding or its export from the nucleus results in retention of target messenger RNAs in the nucleus and reduced levels of the protein products of TAF7-target RNAs. Thus, TAF7, an essential transcription factor, plays a key role in the regulation of RNA translation, thereby potentially connecting these processes. |
| Databáze: | OpenAIRE |
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