Quadruple labeling of brain-stem neurons: a multiple retrograde fluorescent tracer study of axonal collateralization
| Autor: | Akinsegun Akintunde, Donald F. Buxton |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Stilbamidines Red nucleus Central nervous system Amidines Biology Axonal Transport chemistry.chemical_compound Fluorescence microscope medicine Animals Propidium iodide Axon Retrograde direction Fluorescent Dyes Neurons Afferent Pathways General Neuroscience Spinal cord Rats medicine.anatomical_structure Microscopy Fluorescence Spinal Cord nervous system chemistry Biophysics Female Nucleus Neuroscience Brain Stem Propidium |
| Zdroj: | Journal of Neuroscience Methods. 45:15-22 |
| ISSN: | 0165-0270 |
| Popis: | Four different fluorochromes were injected into adjacent cervical spinal cord segments, 1 unique tracer per segment. Each tracer, Fluoro-Gold, Fast Blue, Diamidino Yellow dihydrochloride and Propidium Iodide, was taken up by axonal terminals and transported intra-axonally in a retrograde direction to the cell bodies. Some, though by no means all, of these axons were stem axons with terminals in 2, 3 or 4 of the injected spinal segments. Hence as many as 4 different fluorescent tracers could be discerned simultaneously within individual neuronal somata of origin using fluorescent microscopy. These results extend the possibilities for multiple interconnection determinations within the central nervous system. Specifically, the potential for individual neurons of a nucleus to project collateral branches of a stem axon to as many as 4 different central nervous system nuclei now can be studied simultaneously using these 4 fluorescent tracers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|