Adenovirus-mediated gene transfer in dog prostate: a preclinical study of a relevant model system for gene therapy of human prostatic cancer
| Autor: | M Andrawiss, Patrick Saulnier, Philippe Berthon, Eric J. Kremer, Michel Perricaudet, N D Di Falco, Patrick Devauchelle, Olivier Cussenot, K. Benihoud, J-M Villette, Paule Opolon |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Prostate Cancer and Prostatic Diseases. 2:25-35 |
| ISSN: | 1476-5608 1365-7852 |
| DOI: | 10.1038/sj.pcan.4500278 |
| Popis: | This present study evaluates the potential of adenovirus-mediated gene transfer (AMGT) to the prostate of normal laboratory beagles. Many morphological and histological similarities can be noted between dog and human prostate. Moreover, dogs can spontaneously develop prostate cancer with a clinical and biological outcome identical to that in man. Firstly we showed the capacity of human adenovirus to infect canine prostatic cells in vitro. Secondly, we injected transrectally in the dogs' prostates 2x10(9) plaque forming units of a first generation recombinant adenovirus vector harboring the reporter gene beta-galactosidase (AdRSVbetagal). Seven days after the adenoviral delivery, we observed expression of the transgene in both prostates, and exclusively in epithelial cells. Despite a cellular and a humoral immune response, the infusion appeared safe, since the dogs had no fever and presented no urinary symptoms. This study constitutes the first evaluation of AMGT in dog prostate and provides a basis for gene therapy treatment of prostate carcinoma-bearing patients. |
| Databáze: | OpenAIRE |
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