Expression of alpha-D-mannosidase in man-hamster somatic cell hybrids
| Autor: | Peter H. Ingram, Gail A. P. Bruns, Robert Eisenman, V.M. Regina, Park S. Gerald |
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| Rok vydání: | 1977 |
| Předmět: |
Glucose-6-phosphate isomerase
Cytoplasm Chromosomes Human 19-20 Hamster Neuraminidase Locus (genetics) Biology Hybrid Cells In Vitro Techniques Disaccharidases Biochemistry Isozyme HeLa Chromosome 19 Cricetinae Mannosidases Genetics Animals Humans Molecular Biology Ecology Evolution Behavior and Systematics Glucose-6-Phosphate Isomerase Chromosome Mapping General Medicine Fibroblasts biology.organism_classification Molecular biology Isoenzymes Ploidy Lysosomes HeLa Cells |
| Zdroj: | Biochemical genetics. 15(5-6) |
| ISSN: | 0006-2928 |
| Popis: | Two types of alpha-D-mannosidase isozymes are present in human white blood cells, human diploid fibroblasts, and HeLa cells. One of these (the S isozyme) constitutes the major alpha-D-mannosidase of the human cells, has a pH optimum of 4.4, and is associated with lysosomes. The other (the F isozyme) is most active at pH 6, is acid labile, and is located in the soluble portion of the cytoplasm. The expression of human lysosomal alpha-D-mannosidase was examined in man-hamster hybrid clones, and was found to be concordant with that of phosphohexose isomerase in 54 of 55 primary clones. A locus specifying human lysosomal alpha-D-mannosidase has therfore been assigned to chromosome 19. |
| Databáze: | OpenAIRE |
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