Isolation, fractionation, and some chemical studies on Kleinhovia hospita Linn. seed protein
| Autor: | Amalendu Sinhababu, Milan Chandra Dey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Size-exclusion chromatography
lcsh:TX341-641 Fractionation 01 natural sciences Kleinhovia hospita Glutelin SDS–PAGE parasitic diseases Polyacrylamide gel electrophoresis Gel electrophoresis chemistry.chemical_classification Chromatography biology lcsh:TP368-456 010405 organic chemistry 010401 analytical chemistry Nitrogen solubility biology.organism_classification 0104 chemical sciences Amino acid lcsh:Food processing and manufacture chemistry Sephadex SEM biology.protein lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | International Journal of Food Properties, Vol 21, Iss 1, Pp 510-518 (2018) |
| ISSN: | 1532-2386 1094-2912 |
| Popis: | Protein was extracted from the seeds of Kleinhovia hospita Linn., which is being a non-conventional source Extraction of K. hospita seed protein at various pH values in aqueous solution and at pH 7 of different salt concentrations were done. Fractionation of protein from seeds was performed to separate albumin, globulin, prolamin and glutelin. The amino acid compositions of total protein isolate (TPI) and the fractions were determined. A total of 15 amino acids were identified including 9essential amino acids. Gel filtration by Sephadex G-100 revealed the presence of three components in the total protein isolate. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of TPI and fractions showed different polypeptide bands having molecular weights ranging from 12–42 kDa approximately. Scanning electron microscopic (SEM) study of TPI and fractions revealed the surface topology of the protein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |