Data processing for radial immunodiffusion
| Autor: | Keith M. Cowan, G. Gale Wagner, Rodes Trautman |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Immunodiffusion
Guinea Pigs Immunoglobulins Physical Therapy Sports Therapy and Rehabilitation Immunoglobulin G Antigen-Antibody Reactions Viral Proteins Sample volume Aphthovirus Antigen Molar ratio Methods Animals Antigens Viral Radial immunodiffusion Chromatography biology Computers Chemistry Rehabilitation General Medicine Precipitin Agar Precipitins Models Chemical biology.protein Antibody |
| Zdroj: | Immunochemistry. 8:901-916 |
| ISSN: | 0019-2791 |
| DOI: | 10.1016/0019-2791(71)90429-0 |
| Popis: | The procedure for calculation of the ratio of the number of antibody to antigen molecules initially contained within a precipitin ring is given for both the forward (antigen-in-the-well) and reverse (antibody-in-the-well) methods of radial immunodiffusion from single wells. This molar ratio was determined for three pairs of reagens of the foot-and-mouth disease guinea pig immune system: virus and IgG, virus and IgM, and coat protein antigen and IgG. None of the ratios corresponded to equivalence of the precipitin reactions. Instead, the antibody-antigen ratio was greater by an order of magnitude for the reverse as compared with the forward method. Furthermore, the ratio for the reverse method corresponded roughly to that in extreme antibody excess while the ratio for the forward method was close to the molar ratio in antigen excess. Formulas are developed for data processing that permit correction for wel size and sample volume besides providing internal standard errors of results. These relationships are used in interpreting special experiments showing the dynamic nature of the immunodiffusion process even after the precipitin ring stops growing. |
| Databáze: | OpenAIRE |
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