Utjecaj poliheksametilenova bigvanida na mikroorganizme u korijenskom kanalu zuba
| Autor: | Paris Simeon, Ivana Medvedec Mikić, Arjana Tambić Andrašević, Antonija Tadin, Goranka Prpić-Mehičić, Jurica Matijević |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Colony-forming unit
Veterinary medicine biology Chemistry medicine.medical_treatment Root canal biology.organism_classification Vial Enterococcus faecalis Agar plate chemistry.chemical_compound medicine.anatomical_structure Sodium hypochlorite medicine zubna pulpa komora Pseudomonas aeruginosa Candida albicans korijenski kanal sredstva za ispiranje poliheksametilenov bigvanid natrijev hipoklorit Dental Pulp Cavity Root Canal Irrigants Polyhexamethilen biguanide Sodium Hypochlorite General Dentistry Saline Incubation |
| Zdroj: | Acta stomatologica Croatica : International journal of oral sciences and dental medicine Volume 47 Issue 2 |
| ISSN: | 0001-7019 1846-0410 |
| Popis: | Svrha rada: Željela se ispitati djelotvornost 0,2-postotnog poliheksametilenova bigvanida (PHMB) u eliminaciji Enterococcus faecalis, Pseudomonas aeruginosa i Candida albicans te ga usporediti s učinkovitošću 2,5-postotnog natrijeva hipoklorita (NaOCl). Materijali i metode: Na četrdeset osam jednokorijenskih zuba uklonjeni su krune i apikalni dijelovi te su korijenskih kanali instrumentirani Hedström strugaËima do veličine 40. Šesnaest uzoraka inokulirano je bujonskom kulturom E. faecalis (ATCC 51299), P. aeruginosa (ATCC27853) i C. albicans (klinički izolat). Nakon 48-satne inkubacije i ispiranja fiziološkom otopinom uzeti su uzorci dentina iz korijenskih kanala Hedströmovim iglicama veličine 50 i to prije ispiranja 0,2-postotnim PHMB-om i 2,5-postotnim NaOCl-om i nakon toga postupka. Uzorci su zatim preneseni u epruvete s 2 mL sterilne fiziološke otopine i deseterostrukim razrjeđenjem, te su prebačeni na krvni agar nakon 30 sekundi vorteksiranja. Nakon 48-satne inkubacije na 37 ° C prebrojene su bakterijske kolonije po mililitru (CFU/ml). Za statističku analizu korišten je Kruskal-Wallisov test (p Objective of work: The objective of this study was to test the effectiveness of 0.2% polyhexamethylen biguanide in the elimination of Enterococcus faecalis, Pseudomonas aeruginosa and Candida albicans and compare it with effectiveness of 2.5% sodium hypochlorite. Materials and Methods: Crown and apical portion of forty- eight single-rooted human teeth was removed and the root canals instrumented with Hedström files up to size 40. Sixteen specimens were inoculated with an overnight broth culture of E.faecalis (ATCC 51299), P.aeruginosa (ATCC27853) and C.albicans (clinical isolate). After 48 h incubation and irrigation with saline, dentine samples were collected from root canal walls with Hedström files size 50 before and after irrigation with 0.2% PHMB and 2.5% NaOCl followed by irrigation with neutralizer and saline. Samples were put into vials containing 2 mL of sterile saline and tenfold dilution, and plated on blood agar after 30 s vortexing. Colony forming units (CFU) were counted after 48 h incubation at 37ºC. For statistical analysis Kruskal-Wallis test (p |
| Databáze: | OpenAIRE |
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