In vitro analysis of transneuronal spread of an alphaherpesvirus infection in peripheral nervous system neurons
Autor: | Becket Feierbach, Joseph Goodhouse, Lynn W. Enquist, M. E. Bisher |
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Rok vydání: | 2007 |
Předmět: |
Nervous system
Author's Correction Swine viruses Immunology Pseudorabies Biology Microbiology Cell Line In vitro analysis Viral Envelope Proteins In vivo Postsynaptic potential Live cell imaging Virology Biological neural network medicine Animals Peripheral Nerves Biological Transport biology.organism_classification Herpesvirus 1 Suid Axons Cell biology Virus-Cell Interactions medicine.anatomical_structure Insect Science Peripheral nervous system Synapses Cattle Neuron |
Zdroj: | Journal of virology. 81(13) |
ISSN: | 0022-538X |
Popis: | The neurotropic alphaherpesviruses invade and spread in the nervous system in a directional manner between synaptically connected neurons. Until now, this property has been studied only in living animals and has not been accessible to in vitro analysis. In this study, we describe an in vitro system in which cultured peripheral nervous system neurons are separated from their neuron targets by an isolator chamber ring. Using pseudorabies virus (PRV), an alphaherpesvirus capable of transneuronal spread in neural circuits of many animals, we have recapitulated in vitro all known genetic requirements for retrograde and anterograde transneuronal spread as determined previously in vivo. We show that in vitro transneuronal spread requires intact axons and the presence of the viral proteins gE, gI, and Us9. We also show that transneuronal spread is dependent on the viral glycoprotein gB, which is required for membrane fusion, but not on gD, which is required for extracellular spread. We demonstrate ultrastructural differences between anterograde- and retrograde-traveling virions. Finally, we show live imaging of dynamic fluorescent virion components in axons and postsynaptic target neurons. |
Databáze: | OpenAIRE |
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