Direct chiral separation of almokalant on Chiralcel OD and Chiralpak AD for liquid chromatographic assay of biological samples
| Autor: | Karin Balmér, Per-Olof Lagerström, Bengt-Arne Persson, Sam Larsson |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Tris
Carbamate medicine.medical_treatment Phenylcarbamates Biochemistry Analytical Chemistry Propanolamines chemistry.chemical_compound Amylose medicine Humans Cellulose Chromatography Elution Organic Chemistry Reproducibility of Results Stereoisomerism General Medicine Spectrometry Fluorescence chemistry Almokalant Stereoselectivity Carbamates Anti-Arrhythmia Agents Chiralcel OD Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 631:191-196 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/0021-9673(93)80520-i |
| Popis: | The four isomers of almokalant, a new antiarrhythmic substance under investigation, were separated by liquid chromatography on a Chiralcel OD and a Chiralpak AD column containing cellulose and amylose tris(3,5-dimethylphenylcarbamate), respectively. Both chiral stationary phases separate almokalant into the four isomers, but the retention orders are different if the carbamate is derivatized on cellulose or amylose. The Chiralcel OD column was used for the separation and determination of the isomers in urine at levels down to 100 nmol/l for the first three eluted and 200 nmol/l for the last with a relative standard deviation of less than 15%. The fluorescence response was increased by post-column ionization after stereoselective separation on the Chiralpak AD column. The isomers of almokalant could be determined at levels down to 10 nmol/l in plasma with a relative standard deviation of less than 15%. |
| Databáze: | OpenAIRE |
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